To provide a hybridization probe for analysis of the regulation of rat gonadotropin subunit mRNA levels, an effort was made to isolate a cloned cDNA for the β-subunit of rat FSH (FSHβ). Using a cloned bovine FSHβ cDNA as a hybridization probe, a rat pituitary Xgt10 cDNA library was screened and a single, strongly hybridizing clone identified. The 874 base pair cDNA insert from this clone contains the complete sequence of rat FSHβ including an aminoterminal precursor segment. Hybridization of this cloned cDNA to rat pituitary RNA demonstrated the presence of an approximately 2.0 kilobase RNA species containing FSHβ sequences. Cloned rat cDNA was also used to demonstrate that estrogen treatment of ovariectomized female rats results in de-creases in mRNA concentrations for FSH0 and the β-subunit of LH with somewhat smaller decreases in a-subunit mRNA concentrations. Little or no change was detected in the mRNA for the β-subunit of TSH.
ASJC Scopus subject areas
- Molecular Biology