Molecular cloning and expression of Pgp-1. The mouse homology of the human H-CAM (Hermes) lymphocyte homing receptor

D. F.H. Zhou, J. F. Ding, L. J. Picker, R. F. Bargatze, E. C. Butcher, D. V. Goeddel

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Abstract

Mouse phagocytic glycoprotein-1 (Pgp-1; Ly-24) is a 95-kDa glycoprotein of unknown function that has served as an important T cell/leukocyte differentiation marker. Recent work has suggested that it may be related to a human 85- to 95-kDa glycoprotein (termed variously the Hermes Ag/lymphocyte homing receptor, ECMRIII, P80, and CD44) that is involved in lymphocyte binding to high endothelial venules in the process of lymphocyte homing, and has been implicated in other cell adhesion events. The widespread expression of this molecular class in diverse organ systems suggests a broad role in cellular adhesion, and has led to the unifying designating homing-cellular adhesion molecule (H-CAM). By using human H-CAM cDNA probes, we have isolated a full-length cDNA for the mouse homolog. Comparison of the human and mouse sequences reveals that an N-terminal domain homologous to cartilage proteoglycan core and link proteins, as well as the C-terminal transmembrane and cytoplasmic sequences, are highly conserved (89% and 86% identity, respectively). In contrast, a proximal extracellular domain thought to serve as a target for O-glycosylation and chondroitin sulfate attachment has undergone substantial divergence (only 42% identity). Transient expression of the cDNA in CHO cells followed by immunologic staining confirms that this mouse H-CAM cDNA encodes Pgp-1.1, one of two known Pgp-1 alloantigens.

Original languageEnglish (US)
Pages (from-to)3390-3395
Number of pages6
JournalJournal of Immunology
Volume143
Issue number10
StatePublished - Dec 9 1989

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Zhou, D. F. H., Ding, J. F., Picker, L. J., Bargatze, R. F., Butcher, E. C., & Goeddel, D. V. (1989). Molecular cloning and expression of Pgp-1. The mouse homology of the human H-CAM (Hermes) lymphocyte homing receptor. Journal of Immunology, 143(10), 3390-3395.