Molecular cloning and cell-specific growth characterization of polymorphic variants of type D serogroup 2 Simian retroviruses

Gail Marracci, Nancy A. Avery, Stanley M. Shiigi, Gaila Couch, Heidi Palmer, Kirsten Y. Pilcher, Howard Nichols, Lesley M. Hallick, Michael Axthelm, Curtis Machida

Research output: Contribution to journalArticle

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Abstract

Simian retroviruses (SRVs), the etiological agent of a spontaneous Simian acquired immunodeficiency syndrome, endemically infects large percentages of Asian macaques housed in biomedical research colonies and severely compromises the effective use of these species as a viable research animal. We recently described the molecular cloning of a serogroup 2 SRV, D2/RHE/OR, which causes mild immunosuppression in rhesus macaques. A restriction site variant, D2/RHE/OR/V1, has also been recovered from severely ill animals endemically infected with D2/RHE/OR. We now report the complete nucleotide sequences of D2/RHE/OR and D2/RHE/OR/V1. Both infectious molecular clones retain the genetic structure typical of type D SRVs (5' LTR-gag-prt- pol-env-3' LTR) and encode identically sized 8105-bp proviruses. D2/RHE/OR and D2/RHE/OR/V1 are 99.3% similar at the amine acid level, exhibiting only 17 residue differences, of which 10 are located in the envelope glycoproteins. The molecular clones and reciprocal chimeric viruses were used to assess the contribution of different genetic domains to virus infectivity in a T cell infection assay. These experiments indicate that D2/RHE/OR has a reduced ability to infect specific T cell lines, especially Hut-78 and MT-4 cells, and that the envelope gene is not the sole determinant of in vitro tropism.

Original languageEnglish (US)
Pages (from-to)43-58
Number of pages16
JournalVirology
Volume261
Issue number1
DOIs
StatePublished - Aug 15 1999

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Simian Retroviruses
Mason-Pfizer monkey virus
Molecular Cloning
Simian Acquired Immunodeficiency Syndrome
Clone Cells
Viruses
T-Lymphocytes
Proviruses
Tropism
Genetic Structures
Macaca
Growth
Macaca mulatta
Immunosuppression
Amines
Biomedical Research
Glycoproteins
Cell Line
Acids
Infection

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Molecular cloning and cell-specific growth characterization of polymorphic variants of type D serogroup 2 Simian retroviruses. / Marracci, Gail; Avery, Nancy A.; Shiigi, Stanley M.; Couch, Gaila; Palmer, Heidi; Pilcher, Kirsten Y.; Nichols, Howard; Hallick, Lesley M.; Axthelm, Michael; Machida, Curtis.

In: Virology, Vol. 261, No. 1, 15.08.1999, p. 43-58.

Research output: Contribution to journalArticle

Marracci, Gail ; Avery, Nancy A. ; Shiigi, Stanley M. ; Couch, Gaila ; Palmer, Heidi ; Pilcher, Kirsten Y. ; Nichols, Howard ; Hallick, Lesley M. ; Axthelm, Michael ; Machida, Curtis. / Molecular cloning and cell-specific growth characterization of polymorphic variants of type D serogroup 2 Simian retroviruses. In: Virology. 1999 ; Vol. 261, No. 1. pp. 43-58.
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