TY - JOUR
T1 - Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani
AU - Langford, Chris K.
AU - Ewbank, Shawn A.
AU - Hanson, Sheri S.
AU - Ullman, Buddy
AU - Landfear, Scott M.
N1 - Funding Information:
This research was supported by grants AI25920 (S.M.L.) and AI23682 (B.U.) from the National Institutes of Health and by a grant from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) to S.M.L. S.M.L. also acknowledges a New Investigator Award in Molecular Parasitology from the Burroughs Wellcome Fund. We would like to thank Tom Allen for generous technical help with the PCR procedures used to map the 5' end of the D2 mRNA.
PY - 1992/10
Y1 - 1992/10
N2 - The polymerase chain reaction was used to clone two genes from Leishmania donovani, each of which encodes a member of a superfamily of membrane transporters which include the mammalian facilitative glucose transporters. One of these transporters, designated D2, is similar in sequence and overall structural features to a previously cloned Leishmania transporter Pro-1. Both D2 and Pro-1 are developmentally regulated genes which are expressed primarily in the insect stage of the parasite life cycle. In contrast, the second novel transporter, D1, is structurally quite different from different from either D2 or Pro-1, and its expression is not regulated during the parasite life cycle. All three genes are located on different chromosomes in L. donovani.
AB - The polymerase chain reaction was used to clone two genes from Leishmania donovani, each of which encodes a member of a superfamily of membrane transporters which include the mammalian facilitative glucose transporters. One of these transporters, designated D2, is similar in sequence and overall structural features to a previously cloned Leishmania transporter Pro-1. Both D2 and Pro-1 are developmentally regulated genes which are expressed primarily in the insect stage of the parasite life cycle. In contrast, the second novel transporter, D1, is structurally quite different from different from either D2 or Pro-1, and its expression is not regulated during the parasite life cycle. All three genes are located on different chromosomes in L. donovani.
KW - Developmental regulation
KW - Glucose transporter superfamily
KW - Leishmania
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0026630255&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026630255&partnerID=8YFLogxK
U2 - 10.1016/0166-6851(92)90126-5
DO - 10.1016/0166-6851(92)90126-5
M3 - Article
C2 - 1435876
AN - SCOPUS:0026630255
SN - 0166-6851
VL - 55
SP - 51
EP - 64
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1-2
ER -