Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru

Vilma Bejar, Mercedes Tello, Ruth García, José M. Guevara, Sofia Gonzales, German Vergaray, Esther Valencia, Enma Abanto, Alex Ortega Loayza, Ferry Hagen, Ericson L. Gutierrez

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii. Aims: To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods: A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results: Using conventional PCR, 27 (84.4%) of the isolates were identified as C. neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50%) of the C. neoformans strains were genotype AFLP1, 13 (40.6%) were genotype AFLP1B, 2 (6.3%) were genotype AFLP2, and 1 (3.1%) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C. neoformans are sensitive to these antifungal compounds. Conclusions: In this study we observed that C. neoformans var. grubii (AFLP1 and AFLP1B) and C. neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature.

Original languageEnglish (US)
Pages (from-to)88-92
Number of pages5
JournalRevista Iberoamericana de Micologia
Volume32
Issue number2
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Peru
Genotype
Cryptococcus gattii
Tropical Medicine
Cryptococcus
Cryptococcosis
Mycoses
Fluconazole
Amphotericin B
Retrospective Studies
Polymerase Chain Reaction

Keywords

  • Cryptococcus neoformans
  • Genotype
  • Mating-type
  • Serotyping

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru. / Bejar, Vilma; Tello, Mercedes; García, Ruth; Guevara, José M.; Gonzales, Sofia; Vergaray, German; Valencia, Esther; Abanto, Enma; Ortega Loayza, Alex; Hagen, Ferry; Gutierrez, Ericson L.

In: Revista Iberoamericana de Micologia, Vol. 32, No. 2, 01.04.2015, p. 88-92.

Research output: Contribution to journalArticle

Bejar, V, Tello, M, García, R, Guevara, JM, Gonzales, S, Vergaray, G, Valencia, E, Abanto, E, Ortega Loayza, A, Hagen, F & Gutierrez, EL 2015, 'Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru', Revista Iberoamericana de Micologia, vol. 32, no. 2, pp. 88-92. https://doi.org/10.1016/j.riam.2014.01.005
Bejar, Vilma ; Tello, Mercedes ; García, Ruth ; Guevara, José M. ; Gonzales, Sofia ; Vergaray, German ; Valencia, Esther ; Abanto, Enma ; Ortega Loayza, Alex ; Hagen, Ferry ; Gutierrez, Ericson L. / Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru. In: Revista Iberoamericana de Micologia. 2015 ; Vol. 32, No. 2. pp. 88-92.
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abstract = "Background: Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii. Aims: To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods: A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results: Using conventional PCR, 27 (84.4{\%}) of the isolates were identified as C. neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50{\%}) of the C. neoformans strains were genotype AFLP1, 13 (40.6{\%}) were genotype AFLP1B, 2 (6.3{\%}) were genotype AFLP2, and 1 (3.1{\%}) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C. neoformans are sensitive to these antifungal compounds. Conclusions: In this study we observed that C. neoformans var. grubii (AFLP1 and AFLP1B) and C. neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature.",
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AU - Gonzales, Sofia

AU - Vergaray, German

AU - Valencia, Esther

AU - Abanto, Enma

AU - Ortega Loayza, Alex

AU - Hagen, Ferry

AU - Gutierrez, Ericson L.

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