Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores

Arijit Banerjee, Ellina Mikhailova, Stephen Cheley, Li Qun Gu, Michelle Montoya, Yasuo Nagaoka, Eric Gouaux, Hagan Bayley

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and microdevices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the α-hemolysin (αHL) pore that bind the adapter β-cyclodextrin (βCD) ∼104 times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail.

Original languageEnglish (US)
Pages (from-to)8165-8170
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number18
DOIs
StatePublished - May 4 2010

Fingerprint

Nanopores
Protein Engineering
Porins
Crystallography
Hemolysin Proteins
Cyclodextrins
Biotechnology
DNA Sequence Analysis
Mutagenesis
Binding Sites
X-Rays
Amino Acids
Proteins

Keywords

  • Alpha-hemolysin
  • Single molecule
  • Stochastic sensing
  • Structure
  • Unnatural amino acid

ASJC Scopus subject areas

  • General

Cite this

Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores. / Banerjee, Arijit; Mikhailova, Ellina; Cheley, Stephen; Gu, Li Qun; Montoya, Michelle; Nagaoka, Yasuo; Gouaux, Eric; Bayley, Hagan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 18, 04.05.2010, p. 8165-8170.

Research output: Contribution to journalArticle

Banerjee, Arijit ; Mikhailova, Ellina ; Cheley, Stephen ; Gu, Li Qun ; Montoya, Michelle ; Nagaoka, Yasuo ; Gouaux, Eric ; Bayley, Hagan. / Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 18. pp. 8165-8170.
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