Molecular architecture of the chick vestibular hair bundle

Jung Bum Shin, Jocelyn F. Krey, Ahmed Hassan, Zoltan Metlagel, Andrew N. Tauscher, James M. Pagana, Nicholas E. Sherman, Erin D. Jeffery, Kateri J. Spinelli, Hongyu Zhao, Phillip A. Wilmarth, Dongseok Choi, Larry L. David, Manfred Auer, Peter G. Barr-Gillespie

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Hair bundles of the inner ear have a specialized structure and protein composition that underlies their sensitivity to mechanical stimulation. Using mass spectrometry, we identified and quantified >1,100 proteins, present from a few to 400,000 copies per stereocilium, from purified chick bundles; 336 of these were significantly enriched in bundles. Bundle proteins that we detected have been shown to regulate cytoskeleton structure and dynamics, energy metabolism, phospholipid synthesis and cell signaling. Three-dimensional imaging using electron tomography allowed us to count the number of actin-actin cross-linkers and actin-membrane connectors; these values compared well to those obtained from mass spectrometry. Network analysis revealed several hub proteins, including RDX (radixin) and SLC9A3R2 (NHERF2), which interact with many bundle proteins and may perform functions essential for bundle structure and function. The quantitative mass spectrometry of bundle proteins reported here establishes a framework for future characterization of dynamic processes that shape bundle structure and function.

Original languageEnglish (US)
Pages (from-to)365-374
Number of pages10
JournalNature Neuroscience
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2013

ASJC Scopus subject areas

  • Neuroscience(all)

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    Shin, J. B., Krey, J. F., Hassan, A., Metlagel, Z., Tauscher, A. N., Pagana, J. M., Sherman, N. E., Jeffery, E. D., Spinelli, K. J., Zhao, H., Wilmarth, P. A., Choi, D., David, L. L., Auer, M., & Barr-Gillespie, P. G. (2013). Molecular architecture of the chick vestibular hair bundle. Nature Neuroscience, 16(3), 365-374. https://doi.org/10.1038/nn.3312