Molecular and biochemical elucidation of a cellular phenotype characterized by adenine analogue resistance in the presence of high levels of adenine phosphoribosyltransferase activity

Nada H. Khattar, Gregory E. Cooper, Debra L. DiMartino, Peggy L. Bishop, Mitchell Turker

Research output: Contribution to journalArticle

Abstract

A mouse embryonal carcinoma cell line isolated for resistance to the adenine analogue 2,6-diaminopurine (DAP) was found to have near-wild-type levels of adenine phosphoribosyltransferase (APRT) activity in a cell-free assay. This DAP-resistant (DAPr) cell line, termed H29D1, also exhibited near-wild-type levels of adenine accumulation and the ability to grow in medium containing azaserine and adenine. Growth in this medium requires high levels of intracellular APRT activity. Using the polymerase chain reaction (PCR) and the dideoxy chain termination sequencing technique, an A → G transition was discovered in exon 3 of the aprt gene in H29D1. This mutation resulted in an Arg-to-Gln change at amino acid 87 of the APRT protein that, in turn, resulted in a decreased affinity for adenine. An increased sensitivity of APRT to inhibition by AMP was observed when comparing H29D1 to P19, the parental cell line. Using a transgene containing the A → G mutation, we demonstrated that this mutation is responsible for the biochemical and cellular phenotypes observed for the H29D1 cell line. The approach used in this study provides a definitive method for linking a mutation to a specific cellular phenotype.

Original languageEnglish (US)
Pages (from-to)635-648
Number of pages14
JournalBiochemical Genetics
Volume30
Issue number11
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

adenine phosphoribosyltransferase
Adenine Phosphoribosyltransferase
Adenine
adenine
phenotype
mutation
Cells
cell lines
Phenotype
Cell Line
Mutation
azaserine
Azaserine
Embryonal Carcinoma Stem Cells
Polymerase chain reaction
Adenosine Monophosphate
Transgenes
polymerase chain reaction
transgenes
carcinoma

Keywords

  • adenine phosphoribosyltransferase
  • base-pair substitution
  • mouse
  • polymerase chain reaction
  • structure-function relationship

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Molecular and biochemical elucidation of a cellular phenotype characterized by adenine analogue resistance in the presence of high levels of adenine phosphoribosyltransferase activity. / Khattar, Nada H.; Cooper, Gregory E.; DiMartino, Debra L.; Bishop, Peggy L.; Turker, Mitchell.

In: Biochemical Genetics, Vol. 30, No. 11, 1992, p. 635-648.

Research output: Contribution to journalArticle

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AU - Turker, Mitchell

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