Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates

Ryan M. Pace, Amanda L. Prince, Jun Ma, Benjamin D.W. Belfort, Alexia S. Harvey, Min Hu, Karalee Baquero, Peter Blundell, Diana Takahashi, Tyler Dean, Paul Kievit, Elinor Sullivan, Jacob E. Friedman, Kevin Grove, Kjersti M. Aagaard

Research output: Contribution to journalArticle

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Abstract

Background: We and others have previously shown that alterations in the mammalian gut microbiome are associated with diet, notably early life exposure to a maternal high fat diet (HFD). Here, we aimed to further these studies by examining alterations in the gut microbiome of juvenile Japanese macaques (Macaca fuscata) that were exposed to a maternal HFD, weaned onto a control diet, and later supplemented with a synbiotic comprised of psyllium seed and Enterococcus and Lactobacillus species. Results: Eighteen month old offspring (n = 7) of 36% HFD fed dams were fed a control (14% fat) diet post weaning, then were synbiotic supplemented for 75 days and longitudinal stool and serum samples were obtained. All stool samples were subjected to 16S rRNA metagenomic sequencing, and microbiome profiles and serum lipids and triglycerides were compared to untreated, healthy age matched and diet matched controls (n = 7). Overall, 16S-based metagenomic analysis revealed that supplementation exerted minimal alterations to the gut microbiome including transient increased abundance of Lactobacillus species and decreased abundance of few bacterial genera, including Faecalibacterium and Anaerovibrio. However, serum lipid analysis revealed significant decreases in triglycerides, cholesterol, and LDL (p < 0.05). Nevertheless, supplemented juveniles challenged 4 months later were not protected from HFD-induced gut dysbiosis. Conclusions: Synbiotic supplementation is temporally associated with alterations in the gut microbiome and host lipid profiles of juvenile Japanese macaques that were previously exposed to a maternal HFD. Despite these presumptive temporal benefits, a protective effect against later HFD-challenge gut dysbiosis was not observed.

Original languageEnglish (US)
Article number28
JournalBMC Microbiology
Volume18
Issue number1
DOIs
StatePublished - Apr 5 2018

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Synbiotics
High Fat Diet
Primates
Macaca
Dysbiosis
Diet
Metagenomics
Mothers
Lactobacillus
Lipids
Serum
Psyllium
Microbiota
Enterococcus
Weaning
LDL Cholesterol
Gastrointestinal Microbiome
Seeds
Triglycerides
Fats

Keywords

  • Dysbiosis
  • Microbiome
  • Non-human primate
  • Probiotic, synbiotic

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Pace, R. M., Prince, A. L., Ma, J., Belfort, B. D. W., Harvey, A. S., Hu, M., ... Aagaard, K. M. (2018). Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates. BMC Microbiology, 18(1), [28]. https://doi.org/10.1186/s12866-018-1169-9

Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates. / Pace, Ryan M.; Prince, Amanda L.; Ma, Jun; Belfort, Benjamin D.W.; Harvey, Alexia S.; Hu, Min; Baquero, Karalee; Blundell, Peter; Takahashi, Diana; Dean, Tyler; Kievit, Paul; Sullivan, Elinor; Friedman, Jacob E.; Grove, Kevin; Aagaard, Kjersti M.

In: BMC Microbiology, Vol. 18, No. 1, 28, 05.04.2018.

Research output: Contribution to journalArticle

Pace, RM, Prince, AL, Ma, J, Belfort, BDW, Harvey, AS, Hu, M, Baquero, K, Blundell, P, Takahashi, D, Dean, T, Kievit, P, Sullivan, E, Friedman, JE, Grove, K & Aagaard, KM 2018, 'Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates', BMC Microbiology, vol. 18, no. 1, 28. https://doi.org/10.1186/s12866-018-1169-9
Pace, Ryan M. ; Prince, Amanda L. ; Ma, Jun ; Belfort, Benjamin D.W. ; Harvey, Alexia S. ; Hu, Min ; Baquero, Karalee ; Blundell, Peter ; Takahashi, Diana ; Dean, Tyler ; Kievit, Paul ; Sullivan, Elinor ; Friedman, Jacob E. ; Grove, Kevin ; Aagaard, Kjersti M. / Modulations in the offspring gut microbiome are refractory to postnatal synbiotic supplementation among juvenile primates. In: BMC Microbiology. 2018 ; Vol. 18, No. 1.
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abstract = "Background: We and others have previously shown that alterations in the mammalian gut microbiome are associated with diet, notably early life exposure to a maternal high fat diet (HFD). Here, we aimed to further these studies by examining alterations in the gut microbiome of juvenile Japanese macaques (Macaca fuscata) that were exposed to a maternal HFD, weaned onto a control diet, and later supplemented with a synbiotic comprised of psyllium seed and Enterococcus and Lactobacillus species. Results: Eighteen month old offspring (n = 7) of 36{\%} HFD fed dams were fed a control (14{\%} fat) diet post weaning, then were synbiotic supplemented for 75 days and longitudinal stool and serum samples were obtained. All stool samples were subjected to 16S rRNA metagenomic sequencing, and microbiome profiles and serum lipids and triglycerides were compared to untreated, healthy age matched and diet matched controls (n = 7). Overall, 16S-based metagenomic analysis revealed that supplementation exerted minimal alterations to the gut microbiome including transient increased abundance of Lactobacillus species and decreased abundance of few bacterial genera, including Faecalibacterium and Anaerovibrio. However, serum lipid analysis revealed significant decreases in triglycerides, cholesterol, and LDL (p < 0.05). Nevertheless, supplemented juveniles challenged 4 months later were not protected from HFD-induced gut dysbiosis. Conclusions: Synbiotic supplementation is temporally associated with alterations in the gut microbiome and host lipid profiles of juvenile Japanese macaques that were previously exposed to a maternal HFD. Despite these presumptive temporal benefits, a protective effect against later HFD-challenge gut dysbiosis was not observed.",
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AU - Pace, Ryan M.

AU - Prince, Amanda L.

AU - Ma, Jun

AU - Belfort, Benjamin D.W.

AU - Harvey, Alexia S.

AU - Hu, Min

AU - Baquero, Karalee

AU - Blundell, Peter

AU - Takahashi, Diana

AU - Dean, Tyler

AU - Kievit, Paul

AU - Sullivan, Elinor

AU - Friedman, Jacob E.

AU - Grove, Kevin

AU - Aagaard, Kjersti M.

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N2 - Background: We and others have previously shown that alterations in the mammalian gut microbiome are associated with diet, notably early life exposure to a maternal high fat diet (HFD). Here, we aimed to further these studies by examining alterations in the gut microbiome of juvenile Japanese macaques (Macaca fuscata) that were exposed to a maternal HFD, weaned onto a control diet, and later supplemented with a synbiotic comprised of psyllium seed and Enterococcus and Lactobacillus species. Results: Eighteen month old offspring (n = 7) of 36% HFD fed dams were fed a control (14% fat) diet post weaning, then were synbiotic supplemented for 75 days and longitudinal stool and serum samples were obtained. All stool samples were subjected to 16S rRNA metagenomic sequencing, and microbiome profiles and serum lipids and triglycerides were compared to untreated, healthy age matched and diet matched controls (n = 7). Overall, 16S-based metagenomic analysis revealed that supplementation exerted minimal alterations to the gut microbiome including transient increased abundance of Lactobacillus species and decreased abundance of few bacterial genera, including Faecalibacterium and Anaerovibrio. However, serum lipid analysis revealed significant decreases in triglycerides, cholesterol, and LDL (p < 0.05). Nevertheless, supplemented juveniles challenged 4 months later were not protected from HFD-induced gut dysbiosis. Conclusions: Synbiotic supplementation is temporally associated with alterations in the gut microbiome and host lipid profiles of juvenile Japanese macaques that were previously exposed to a maternal HFD. Despite these presumptive temporal benefits, a protective effect against later HFD-challenge gut dysbiosis was not observed.

AB - Background: We and others have previously shown that alterations in the mammalian gut microbiome are associated with diet, notably early life exposure to a maternal high fat diet (HFD). Here, we aimed to further these studies by examining alterations in the gut microbiome of juvenile Japanese macaques (Macaca fuscata) that were exposed to a maternal HFD, weaned onto a control diet, and later supplemented with a synbiotic comprised of psyllium seed and Enterococcus and Lactobacillus species. Results: Eighteen month old offspring (n = 7) of 36% HFD fed dams were fed a control (14% fat) diet post weaning, then were synbiotic supplemented for 75 days and longitudinal stool and serum samples were obtained. All stool samples were subjected to 16S rRNA metagenomic sequencing, and microbiome profiles and serum lipids and triglycerides were compared to untreated, healthy age matched and diet matched controls (n = 7). Overall, 16S-based metagenomic analysis revealed that supplementation exerted minimal alterations to the gut microbiome including transient increased abundance of Lactobacillus species and decreased abundance of few bacterial genera, including Faecalibacterium and Anaerovibrio. However, serum lipid analysis revealed significant decreases in triglycerides, cholesterol, and LDL (p < 0.05). Nevertheless, supplemented juveniles challenged 4 months later were not protected from HFD-induced gut dysbiosis. Conclusions: Synbiotic supplementation is temporally associated with alterations in the gut microbiome and host lipid profiles of juvenile Japanese macaques that were previously exposed to a maternal HFD. Despite these presumptive temporal benefits, a protective effect against later HFD-challenge gut dysbiosis was not observed.

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