Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents

Carolina Bosso André, Pedro Luiz Rosalen, L. C.C. Galvão, Bruna Marin Fronza, Glaucia Maria Bovi Ambrosano, Jack Ferracane, Marcelo Giannini

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods: Apigenin (1. mM) and tt-Farnesol (5. mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115. h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results: Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin + tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Significance: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.

Original languageEnglish (US)
JournalDental Materials
DOIs
StateAccepted/In press - 2017

Fingerprint

Dental Cements
Apigenin
Streptococcus mutans
Farnesol
Virulence
Adhesives
Polysaccharides
Modulation
Biofilms
Dentin
Proteins
Hydroxyapatite
Microbial Viability
Microorganisms
Weights and Measures
Restoration
Adhesion
Resins
Alkalies
Physical properties

Keywords

  • Apigenin
  • Bacterial virulence
  • Caries
  • Dental adhesives
  • tt-Farnesol

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

André, C. B., Rosalen, P. L., Galvão, L. C. C., Fronza, B. M., Ambrosano, G. M. B., Ferracane, J., & Giannini, M. (Accepted/In press). Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents. Dental Materials. https://doi.org/10.1016/j.dental.2017.07.006

Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents. / André, Carolina Bosso; Rosalen, Pedro Luiz; Galvão, L. C.C.; Fronza, Bruna Marin; Ambrosano, Glaucia Maria Bovi; Ferracane, Jack; Giannini, Marcelo.

In: Dental Materials, 2017.

Research output: Contribution to journalArticle

André, Carolina Bosso ; Rosalen, Pedro Luiz ; Galvão, L. C.C. ; Fronza, Bruna Marin ; Ambrosano, Glaucia Maria Bovi ; Ferracane, Jack ; Giannini, Marcelo. / Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents. In: Dental Materials. 2017.
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AU - André, Carolina Bosso

AU - Rosalen, Pedro Luiz

AU - Galvão, L. C.C.

AU - Fronza, Bruna Marin

AU - Ambrosano, Glaucia Maria Bovi

AU - Ferracane, Jack

AU - Giannini, Marcelo

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N2 - Objectives: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods: Apigenin (1. mM) and tt-Farnesol (5. mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115. h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results: Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin + tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Significance: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.

AB - Objectives: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods: Apigenin (1. mM) and tt-Farnesol (5. mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115. h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results: Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin + tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Significance: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.

KW - Apigenin

KW - Bacterial virulence

KW - Caries

KW - Dental adhesives

KW - tt-Farnesol

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