Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents

Carolina Bosso André, Pedro Luiz Rosalen, L. C.C. Galvão, Bruna Marin Fronza, Glaucia Maria Bovi Ambrosano, Jack Ferracane, Marcelo Giannini

Research output: Contribution to journalArticle

7 Scopus citations


Objectives: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods: Apigenin (1. mM) and tt-Farnesol (5. mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115. h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results: Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin + tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Significance: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.

Original languageEnglish (US)
JournalDental Materials
StateAccepted/In press - 2017


  • Apigenin
  • Bacterial virulence
  • Caries
  • Dental adhesives
  • tt-Farnesol

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

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    André, C. B., Rosalen, P. L., Galvão, L. C. C., Fronza, B. M., Ambrosano, G. M. B., Ferracane, J., & Giannini, M. (Accepted/In press). Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents. Dental Materials.