Modulation of sodium-channel mRNA levels in rat skeletal muscle.

S. S. Cooperman, S. A. Grubman, R. L. Barchi, R. H. Goodman, G. Mandel

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Abstract

Action potentials in many types of excitable cells result from changes in permeability to Na ions. Although these permeability changes in nerve and muscle are mediated by voltage-gated Na channels that are functionally similar, we found that the Na-channel gene expressed in skeletal muscle is different from the genes coding for two Na channels (type I and type II) in brain. Despite the structural differences between muscle and brain Na-channel genes, a cDNA clone derived from rat brain hybridizes to skeletal muscle Na-channel mRNA of approximately 9.5 kilobases. We used this cDNA probe to measure changes in Na-channel mRNA levels in skeletal muscle during development and following denervation. By blot hybridization analysis of electrophoretically fractionated RNA, we found that Na-channel mRNA can be detected as early as embryonic day 17 and that mRNA levels increase 2-fold between birth and postnatal day 35. Denervation of adult muscle causes a further 2- to 3-fold increase in muscle Na-channel mRNA levels, suggesting that expression of Na-channel genes in fast-twitch muscle may be regulated by the state of innervation.

Original languageEnglish (US)
Pages (from-to)8721-8725
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number23
DOIs
StatePublished - Dec 1987

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