Modulation of sodium-channel mRNA levels in rat skeletal muscle.

S. S. Cooperman, S. A. Grubman, R. L. Barchi, Richard Goodman, Gail Mandel

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Action potentials in many types of excitable cells result from changes in permeability to Na ions. Although these permeability changes in nerve and muscle are mediated by voltage-gated Na channels that are functionally similar, we found that the Na-channel gene expressed in skeletal muscle is different from the genes coding for two Na channels (type I and type II) in brain. Despite the structural differences between muscle and brain Na-channel genes, a cDNA clone derived from rat brain hybridizes to skeletal muscle Na-channel mRNA of approximately 9.5 kilobases. We used this cDNA probe to measure changes in Na-channel mRNA levels in skeletal muscle during development and following denervation. By blot hybridization analysis of electrophoretically fractionated RNA, we found that Na-channel mRNA can be detected as early as embryonic day 17 and that mRNA levels increase 2-fold between birth and postnatal day 35. Denervation of adult muscle causes a further 2- to 3-fold increase in muscle Na-channel mRNA levels, suggesting that expression of Na-channel genes in fast-twitch muscle may be regulated by the state of innervation.

Original languageEnglish (US)
Pages (from-to)8721-8725
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number23
StatePublished - Dec 1987
Externally publishedYes

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Sodium Channels
Skeletal Muscle
Messenger RNA
Muscles
Genes
Permeability
Brain
Complementary DNA
Muscle Denervation
Muscle Development
Denervation
Action Potentials
Clone Cells
Parturition
RNA
Ions

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Modulation of sodium-channel mRNA levels in rat skeletal muscle. / Cooperman, S. S.; Grubman, S. A.; Barchi, R. L.; Goodman, Richard; Mandel, Gail.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 23, 12.1987, p. 8721-8725.

Research output: Contribution to journalArticle

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