Modulation of progestin secretion in ovarian cells by 17β-hydroxy-5α-androstan-3-one (dihydrotestosterone): A direct demonstration in monolayer culture

These studies with a monolayer system of porcine granulosa cells provide a direct demonstration of the ability of androgen to stimulate progestin secretion by ovarian cells. A preferential action of the more potent androgens, dihydrotestosterone and testosterone, was shown but only dihydrotestosterone demonstrated the capacity to stimulate progestin secretion throughout the culture period. Estradiol 17-β markedly depressed progestin synthesis. The results suggest a modulatory role for androgens in the development of full steroidogenic potential by ovarian granulosa cells during follicular development.