Modulation of myosin heavy chains in rat laryngeal muscle

Akihiro Shiotani, Hideki Nakagawa, Paul Flint

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objectives: To test the hypothesis that myosin heavy chain (MHC) composition is a biological marker indicative of appropriate and functional reinnervation. Study Design: Age-matched adult rats were randomized for prospective study under three experimental conditions. Methods: In adult rats, three experimental conditions were surgically created, including transient recurrent laryngeal nerve (RLN) crush injury, RLN transection and repair, and cricoarytenoid joint fixation with intact RLN. Animals were survived for 30, 90, and 180 days. At each interval, vocal fold mobility was assessed by rigid microlaryngoscopy. Laryngeal electromyography (EMG) was performed before euthanasia. The thyroarytenoid and posterior cricoarytenoid muscles were then excised, each muscle was processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and MHC composition was determined. Results: Thirty days after nerve crush injury, three of six animals regained vocal fold mobility and normal MHC composition. Impaired vocal fold motion in three of six animals was associated with MHC composition characteristic of denervation. At 90 and 180 days, normal vocal fold motion and normal MHC composition were observed in all animals. Following nerve transection and repair, impaired vocal fold motion and MHC composition characteristic of denervation were observed in all animals, despite evidence of reinnervation on EMG. Following joint fixation, alteration in MHC composition consistent with denervation was observed only at 30 days, as was evident in the nerve crush model. Conclusion: Temporary injury and vocal fold immobilization result in transient shifts in MHC composition. Nerve transection and repair result in persistent alteration of MHC composition and vocal fold dysfunction. The expression of normal MHC composition is dependent on the condition of appropriate neural contact and functional reinnervation.

Original languageEnglish (US)
Pages (from-to)472-477
Number of pages6
JournalLaryngoscope
Volume111
Issue number3
StatePublished - 2001
Externally publishedYes

Fingerprint

Laryngeal Muscles
Myosin Heavy Chains
Vocal Cords
Nerve Crush
Denervation
Recurrent Laryngeal Nerve Injuries
Electromyography
Joints
Recurrent Laryngeal Nerve
Euthanasia
Sodium Dodecyl Sulfate
Immobilization
Polyacrylamide Gel Electrophoresis
Biomarkers
Prospective Studies

Keywords

  • Cricoarytenoid joint fixation
  • Myosin heavy chain
  • Nerve injury
  • Rat larynx
  • Recurrent laryngeal nerve

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Shiotani, A., Nakagawa, H., & Flint, P. (2001). Modulation of myosin heavy chains in rat laryngeal muscle. Laryngoscope, 111(3), 472-477.

Modulation of myosin heavy chains in rat laryngeal muscle. / Shiotani, Akihiro; Nakagawa, Hideki; Flint, Paul.

In: Laryngoscope, Vol. 111, No. 3, 2001, p. 472-477.

Research output: Contribution to journalArticle

Shiotani, A, Nakagawa, H & Flint, P 2001, 'Modulation of myosin heavy chains in rat laryngeal muscle', Laryngoscope, vol. 111, no. 3, pp. 472-477.
Shiotani, Akihiro ; Nakagawa, Hideki ; Flint, Paul. / Modulation of myosin heavy chains in rat laryngeal muscle. In: Laryngoscope. 2001 ; Vol. 111, No. 3. pp. 472-477.
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