Modulation of Bcl-2 expression

A potential component of estrogen protection in NT2 neurons

Cherie A. Singer, Keith L. Rogers, Daniel Dorsa

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Neuroprotective effects of estrogen have been demonstrated against a variety of cytotoxic insults. We present data here addressing a possible mechanism of estrogen neuroprotection in the human teratocarcinoma cell line NT2 terminally differentiated to a neuronal phenotype. Cell death induced by H2O2 or glutamate results in a dose-dependent cell death of NT2 neurons, while 24 h of estrogen pretreatment significantly enhances neuronal viability. Bcl-2 expression has been shown to reduce oxidative stress and prevent cell death. In NT2 neurons, Bcl-2 levels are dramatically elevated upon differentiation and are further enhanced with estrogen treatment. These results suggest that neuroprotective effects of estrogen may be related to increases in Bcl-2 expression.

Original languageEnglish (US)
Pages (from-to)2565-2568
Number of pages4
JournalNeuroReport
Volume9
Issue number11
StatePublished - Aug 3 1998
Externally publishedYes

Fingerprint

Estrogens
Neurons
Cell Death
Neuroprotective Agents
Teratocarcinoma
Glutamic Acid
Oxidative Stress
Phenotype
Cell Line

Keywords

  • Bcl-2
  • Estrogen
  • Glutamate
  • Neuroprotection
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Modulation of Bcl-2 expression : A potential component of estrogen protection in NT2 neurons. / Singer, Cherie A.; Rogers, Keith L.; Dorsa, Daniel.

In: NeuroReport, Vol. 9, No. 11, 03.08.1998, p. 2565-2568.

Research output: Contribution to journalArticle

Singer, Cherie A. ; Rogers, Keith L. ; Dorsa, Daniel. / Modulation of Bcl-2 expression : A potential component of estrogen protection in NT2 neurons. In: NeuroReport. 1998 ; Vol. 9, No. 11. pp. 2565-2568.
@article{537eafd12d164f648c30d557a8fe6989,
title = "Modulation of Bcl-2 expression: A potential component of estrogen protection in NT2 neurons",
abstract = "Neuroprotective effects of estrogen have been demonstrated against a variety of cytotoxic insults. We present data here addressing a possible mechanism of estrogen neuroprotection in the human teratocarcinoma cell line NT2 terminally differentiated to a neuronal phenotype. Cell death induced by H2O2 or glutamate results in a dose-dependent cell death of NT2 neurons, while 24 h of estrogen pretreatment significantly enhances neuronal viability. Bcl-2 expression has been shown to reduce oxidative stress and prevent cell death. In NT2 neurons, Bcl-2 levels are dramatically elevated upon differentiation and are further enhanced with estrogen treatment. These results suggest that neuroprotective effects of estrogen may be related to increases in Bcl-2 expression.",
keywords = "Bcl-2, Estrogen, Glutamate, Neuroprotection, Oxidative stress",
author = "Singer, {Cherie A.} and Rogers, {Keith L.} and Daniel Dorsa",
year = "1998",
month = "8",
day = "3",
language = "English (US)",
volume = "9",
pages = "2565--2568",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "11",

}

TY - JOUR

T1 - Modulation of Bcl-2 expression

T2 - A potential component of estrogen protection in NT2 neurons

AU - Singer, Cherie A.

AU - Rogers, Keith L.

AU - Dorsa, Daniel

PY - 1998/8/3

Y1 - 1998/8/3

N2 - Neuroprotective effects of estrogen have been demonstrated against a variety of cytotoxic insults. We present data here addressing a possible mechanism of estrogen neuroprotection in the human teratocarcinoma cell line NT2 terminally differentiated to a neuronal phenotype. Cell death induced by H2O2 or glutamate results in a dose-dependent cell death of NT2 neurons, while 24 h of estrogen pretreatment significantly enhances neuronal viability. Bcl-2 expression has been shown to reduce oxidative stress and prevent cell death. In NT2 neurons, Bcl-2 levels are dramatically elevated upon differentiation and are further enhanced with estrogen treatment. These results suggest that neuroprotective effects of estrogen may be related to increases in Bcl-2 expression.

AB - Neuroprotective effects of estrogen have been demonstrated against a variety of cytotoxic insults. We present data here addressing a possible mechanism of estrogen neuroprotection in the human teratocarcinoma cell line NT2 terminally differentiated to a neuronal phenotype. Cell death induced by H2O2 or glutamate results in a dose-dependent cell death of NT2 neurons, while 24 h of estrogen pretreatment significantly enhances neuronal viability. Bcl-2 expression has been shown to reduce oxidative stress and prevent cell death. In NT2 neurons, Bcl-2 levels are dramatically elevated upon differentiation and are further enhanced with estrogen treatment. These results suggest that neuroprotective effects of estrogen may be related to increases in Bcl-2 expression.

KW - Bcl-2

KW - Estrogen

KW - Glutamate

KW - Neuroprotection

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=0032479933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032479933&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 2565

EP - 2568

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 11

ER -