Modulated discharge of Purkinje and stellate cells persists after unilateral loss of vestibular primary afferent mossy fibers in mice

N. H. Barmack, V. Yakhnitsa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cerebellar Purkinje cells are excited by two afferent pathways: climbing and mossy fibers. Climbing fibers evoke large "complex spikes" (CSs) that discharge at low frequencies. Mossy fibers synapse on granule cells whose parallel fibers excite Purkinje cells and may contribute to the genesis of "simple spikes" (SSs). Both afferent systems convey vestibular information to folia 9c-10. After making a unilateral labyrinthectomy (UL) in mice, we tested how the discharge of CSs and SSs was changed by the loss of primary vestibular afferent mossy fibers during sinusoidal roll tilt. We recorded from cells identified by juxtacellular neurobiotin labeling. The UL preferentially reduced vestibular modulation of CSs and SSs in folia 8-10 contralateral to the UL. The effects of a UL on Purkinje cell discharge were similar in folia 9c-10, to which vestibular primary afferents project, and in folia 8-9a, to which they do not project, suggesting that vestibular primary afferent mossy fibers were not responsible for the ULinduced alteration of SS discharge. UL also induced reduced vestibular modulation of stellate cell discharge contralateral to the UL. We attribute the decreased modulation to reduced vestibular modulation of climbing fibers. In summary, climbing fibers modulate CSs directly and SSs indirectly through activation of stellate cells. Whereas vestibular primary afferent mossy fibers cannot account for the modulated discharge of SSs or stellate cells, the nonspecific excitation of Purkinje cells by parallel fibers may set an operating point about which the discharges of SSs are sculpted by climbing fibers.

Original languageEnglish (US)
Pages (from-to)2257-2274
Number of pages18
JournalJournal of Neurophysiology
Volume110
Issue number10
DOIs
StatePublished - Nov 15 2013

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Purkinje Cells
Afferent Pathways
Synapses

Keywords

  • Cerebellum
  • Complex spike
  • Golgi cell
  • Labyrinthectomy
  • Purkinje cell
  • Simple spike
  • Stellate cell

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Modulated discharge of Purkinje and stellate cells persists after unilateral loss of vestibular primary afferent mossy fibers in mice. / Barmack, N. H.; Yakhnitsa, V.

In: Journal of Neurophysiology, Vol. 110, No. 10, 15.11.2013, p. 2257-2274.

Research output: Contribution to journalArticle

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