Mn(II) oxidation is catalyzed by heme peroxidases in "Aurantimonas manganoxydans" strain SI85-9A1 and Erythrobacter sp. strain SD-21

C. R. Anderson, H. A. Johnson, N. Caputo, R. E. Davis, J. W. Torpey, Bradley Tebo

Research output: Contribution to journalArticle

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Abstract

A new type of manganese-oxidizing enzyme has been identified in two alphaproteobacteria, "Aurantimonas manganoxydans" strain SI85-9A1 and Erythrobacter sp. strain SD-21. These proteins were identified by tandem mass spectrometry of manganese-oxidizing bands visualized by native polyacrylamide gel electrophoresis in-gel activity assays and fast protein liquid chromatography-purified proteins. Proteins of both alphaproteobacteria contain animal heme peroxidase and hemolysin-type calcium binding domains, with the 350-kDa active Mn-oxidizing protein of A. manganoxydans containing stainable heme. The addition of both Ca2+ ions and H2O2 to the enriched protein from Aurantimonas increased manganese oxidation activity 5.9-fold, and the highest activity recorded was 700 μM min-1 mg-1. Mn(II) is oxidized to Mn(IV) via an Mn(III) intermediate, which is consistent with known manganese peroxidase activity in fungi. The Mn-oxidizing protein in Erythrobacter sp. strain SD-21 is 225 kDa and contains only one peroxidase domain with strong homology to the first 2,000 amino acids of the peroxidase protein from A. manganoxydans. The heme peroxidase has tentatively been named MopA (manganese-oxidizing peroxidase) and sheds new light on the molecular mechanism of Mn oxidation in prokaryotes.

Original languageEnglish (US)
Pages (from-to)4130-4138
Number of pages9
JournalApplied and Environmental Microbiology
Volume75
Issue number12
DOIs
StatePublished - Jun 2009

Fingerprint

Sphingomonadaceae
Erythrobacter
Peroxidases
Heme
peroxidase
manganese peroxidase
oxidation
Peroxidase
protein
manganese
Manganese
Alphaproteobacteria
Proteins
proteins
Staphylococcal Protein A
alpha-Proteobacteria
Native Polyacrylamide Gel Electrophoresis
Hemolysin Proteins
gel
Tandem Mass Spectrometry

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

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Mn(II) oxidation is catalyzed by heme peroxidases in "Aurantimonas manganoxydans" strain SI85-9A1 and Erythrobacter sp. strain SD-21. / Anderson, C. R.; Johnson, H. A.; Caputo, N.; Davis, R. E.; Torpey, J. W.; Tebo, Bradley.

In: Applied and Environmental Microbiology, Vol. 75, No. 12, 06.2009, p. 4130-4138.

Research output: Contribution to journalArticle

Anderson, C. R. ; Johnson, H. A. ; Caputo, N. ; Davis, R. E. ; Torpey, J. W. ; Tebo, Bradley. / Mn(II) oxidation is catalyzed by heme peroxidases in "Aurantimonas manganoxydans" strain SI85-9A1 and Erythrobacter sp. strain SD-21. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 12. pp. 4130-4138.
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