MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast

Tomas A. Prolla, Qishen Pang, Eric Alani, Richard D. Kolodner, Robert (Mike) Liskay

Research output: Contribution to journalArticle

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Abstract

The discovery that mutations in DNA mismatch repair genes can cause hereditary nonpolyposis colorectal cancer has stimulated interest in understanding the mechanism of DNA mismatch repair in eukaryotes. In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins. Experiments revealed that the yeast MLH1 and PMS1 proteins physically associate, possibly forming a heterodimer, and that MLH1 and PMS1 act in concert to bind a MSH2-heteroduplex complex containing a G-T mismatch. Thus, MSH2, MLH1, and PMS1 are likely to form a ternary complex during the initiation of eukaryotic DNA mismatch repair.

Original languageEnglish (US)
Pages (from-to)1091-1093
Number of pages3
JournalScience
Volume265
Issue number5175
StatePublished - Aug 19 1994

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DNA Mismatch Repair
Yeasts
Hereditary Nonpolyposis Colorectal Neoplasms
Fungal Proteins
Eukaryota
Saccharomyces cerevisiae
Mutation
Genes
S cerevisiae PMS1 protein
MutL Protein Homolog 1

ASJC Scopus subject areas

  • General

Cite this

Prolla, T. A., Pang, Q., Alani, E., Kolodner, R. D., & Liskay, R. M. (1994). MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science, 265(5175), 1091-1093.

MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. / Prolla, Tomas A.; Pang, Qishen; Alani, Eric; Kolodner, Richard D.; Liskay, Robert (Mike).

In: Science, Vol. 265, No. 5175, 19.08.1994, p. 1091-1093.

Research output: Contribution to journalArticle

Prolla, TA, Pang, Q, Alani, E, Kolodner, RD & Liskay, RM 1994, 'MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast', Science, vol. 265, no. 5175, pp. 1091-1093.
Prolla TA, Pang Q, Alani E, Kolodner RD, Liskay RM. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994 Aug 19;265(5175):1091-1093.
Prolla, Tomas A. ; Pang, Qishen ; Alani, Eric ; Kolodner, Richard D. ; Liskay, Robert (Mike). / MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. In: Science. 1994 ; Vol. 265, No. 5175. pp. 1091-1093.
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