TY - JOUR
T1 - MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast
AU - Prolla, Tomas A.
AU - Pang, Qishen
AU - Alani, Eric
AU - Kolodner, Richard D.
AU - Liskay, R. Michael
PY - 1994/8/19
Y1 - 1994/8/19
N2 - The discovery that mutations in DNA mismatch repair genes can cause hereditary nonpolyposis colorectal cancer has stimulated interest in understanding the mechanism of DNA mismatch repair in eukaryotes. In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins. Experiments revealed that the yeast MLH1 and PMS1 proteins physically associate, possibly forming a heterodimer, and that MLH1 and PMS1 act in concert to bind a MSH2-heteroduplex complex containing a G-T mismatch. Thus, MSH2, MLH1, and PMS1 are likely to form a ternary complex during the initiation of eukaryotic DNA mismatch repair.
AB - The discovery that mutations in DNA mismatch repair genes can cause hereditary nonpolyposis colorectal cancer has stimulated interest in understanding the mechanism of DNA mismatch repair in eukaryotes. In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins. Experiments revealed that the yeast MLH1 and PMS1 proteins physically associate, possibly forming a heterodimer, and that MLH1 and PMS1 act in concert to bind a MSH2-heteroduplex complex containing a G-T mismatch. Thus, MSH2, MLH1, and PMS1 are likely to form a ternary complex during the initiation of eukaryotic DNA mismatch repair.
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U2 - 10.1126/science.8066446
DO - 10.1126/science.8066446
M3 - Article
C2 - 8066446
AN - SCOPUS:0028128601
SN - 0036-8075
VL - 265
SP - 1091
EP - 1093
JO - Science
JF - Science
IS - 5175
ER -