TY - JOUR
T1 - Mixtures of benomyl, pirimiphos-methyl, dimethoate, diazinon and azinphos-methyl affect protein synthesis in HL-60 cells differently
AU - Marinovich, Marina
AU - Guizzetti, Marina
AU - Galli, Corrado L.
N1 - Funding Information:
This study was partially supported by project CNR 'FATMA, SP Qualit/l dell'am-biente e salute'. The authors thank Mrs. Helen Downess for kindly revising the manuscript.
PY - 1994
Y1 - 1994
N2 - Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of protein synthesis in HL-60 cells at 24 h, at 60 and 40 μg/ml medium, respectively. Dimethoate and pirimiphos were not active up to 100 μg/ml. Benomyl strongly inhibited protein synthesis at 50 μg/ml and the polymerisation of actin to give cytoskeletal microfilaments (F-actin) at 30 μg/ml. Mixtures of benomylpirimiphos and dimethoate azinphos-diazinon were also investigated. Pirimiphos, when present in equal concentration, antagonized the inhibitory effect of benomyl on protein synthesis at 4 h, but not at 24 h. The effect of the other insecticide mixture on the same parameter was greater than that of the two active components, diazinon and azinphos given singly.
AB - Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of protein synthesis in HL-60 cells at 24 h, at 60 and 40 μg/ml medium, respectively. Dimethoate and pirimiphos were not active up to 100 μg/ml. Benomyl strongly inhibited protein synthesis at 50 μg/ml and the polymerisation of actin to give cytoskeletal microfilaments (F-actin) at 30 μg/ml. Mixtures of benomylpirimiphos and dimethoate azinphos-diazinon were also investigated. Pirimiphos, when present in equal concentration, antagonized the inhibitory effect of benomyl on protein synthesis at 4 h, but not at 24 h. The effect of the other insecticide mixture on the same parameter was greater than that of the two active components, diazinon and azinphos given singly.
KW - Actin
KW - In vitro toxicity
KW - Mixtures
KW - Pesticides
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U2 - 10.1016/0300-483X(94)90036-1
DO - 10.1016/0300-483X(94)90036-1
M3 - Article
C2 - 7801320
AN - SCOPUS:0028154793
SN - 0300-483X
VL - 94
SP - 173
EP - 185
JO - Toxicology
JF - Toxicology
IS - 1-3
ER -