Mixtures of benomyl, pirimiphos-methyl, dimethoate, diazinon and azinphos-methyl affect protein synthesis in HL-60 cells differently

Marina Marinovich, Marina Guizzetti, Corrado L. Galli

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of protein synthesis in HL-60 cells at 24 h, at 60 and 40 μg/ml medium, respectively. Dimethoate and pirimiphos were not active up to 100 μg/ml. Benomyl strongly inhibited protein synthesis at 50 μg/ml and the polymerisation of actin to give cytoskeletal microfilaments (F-actin) at 30 μg/ml. Mixtures of benomylpirimiphos and dimethoate azinphos-diazinon were also investigated. Pirimiphos, when present in equal concentration, antagonized the inhibitory effect of benomyl on protein synthesis at 4 h, but not at 24 h. The effect of the other insecticide mixture on the same parameter was greater than that of the two active components, diazinon and azinphos given singly.

Original languageEnglish (US)
Pages (from-to)173-185
Number of pages13
JournalToxicology
Volume94
Issue number1-3
DOIs
StatePublished - Jan 1 1994

Keywords

  • Actin
  • In vitro toxicity
  • Mixtures
  • Pesticides

ASJC Scopus subject areas

  • Toxicology

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