Mitochondrial replacement therapy in reproductive medicine

Don P. Wolf, Nargiz Mitalipov, Shoukhrat Mitalipov

    Research output: Contribution to journalReview article

    74 Scopus citations

    Abstract

    Mitochondrial dysfunction is implicated in disease and age-related infertility. Mitochondrial replacement therapies (MRT) in oocytes or zygotes, such as pronuclear (PNT), spindle (ST), or polar body (PBT) transfer, could prevent second-generation transmission of mitochondrial DNA (mtDNA) defects. PNT, associated with high levels of mtDNA carryover in mice but low levels in human embryos, carries ethical issues secondary to donor embryo destruction. ST, developed in primates, supports normal development to adults and low mtDNA carryover. PBT in mice, coupled with PN or ST, may increase the yield of reconstructed embryos with low mtDNA carryover. MRT also offers replacement of the deficient cytoplasm in oocytes from older patients, with the expectation of high pregnancy rates following in vitro fertilization.

    Original languageEnglish (US)
    Pages (from-to)68-76
    Number of pages9
    JournalTrends in Molecular Medicine
    Volume21
    Issue number2
    DOIs
    StatePublished - Feb 1 2015

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    Keywords

    • Female infertility
    • Mitochondria
    • Mitochondrial DNA
    • Mitochondrial replacement therapy

    ASJC Scopus subject areas

    • Molecular Medicine
    • Molecular Biology

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