Mitochondrial genome inheritance and replacement in the human germline

Don P. Wolf, Tomonari Hayama, Shoukhrat Mitalipov

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Mitochondria, the ubiquitous power packs in nearly every eukaryotic cell, contain their own DNA, known as mtDNA, which is inherited exclusively from the mother. The number of mitochondrial genomes varies depending on the cell's energy needs. The mature oocyte contains the highest number of mitochondria of any cell type, although there is little if any mtDNA replication after fertilization until the embryo implants. This has potential repercussions for mitochondrial replacement therapy (MRT; see description of currently employed methods below) used to prevent the transmission of mtDNA-based disorders. If only a few mitochondria with defective mtDNA are left in the embryo and undergo extensive replication, it might therefore thwart the purpose of MRT. In order to improve the safety and efficacy of this experimental therapy, we need a better understanding of how and which mtDNA is tagged for replication versus transcription after fertilization of the oocyte.

    Original languageEnglish (US)
    JournalEMBO Journal
    DOIs
    StateAccepted/In press - 2017

    Fingerprint

    Mitochondrial Genome
    Mitochondrial Genes
    Mitochondrial DNA
    Genes
    Mitochondria
    Fertilization
    Oocytes
    Embryonic Structures
    Investigational Therapies
    Eukaryotic Cells
    Transcription
    Safety
    DNA

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Immunology and Microbiology(all)
    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Mitochondrial genome inheritance and replacement in the human germline. / Wolf, Don P.; Hayama, Tomonari; Mitalipov, Shoukhrat.

    In: EMBO Journal, 2017.

    Research output: Contribution to journalArticle

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