Mitochondrial genome inheritance and replacement in the human germline

Don P. Wolf, Tomonari Hayama, Shoukhrat Mitalipov

    Research output: Contribution to journalArticle

    11 Scopus citations

    Abstract

    Mitochondria, the ubiquitous power packs in nearly every eukaryotic cell, contain their own DNA, known as mtDNA, which is inherited exclusively from the mother. The number of mitochondrial genomes varies depending on the cell's energy needs. The mature oocyte contains the highest number of mitochondria of any cell type, although there is little if any mtDNA replication after fertilization until the embryo implants. This has potential repercussions for mitochondrial replacement therapy (MRT; see description of currently employed methods below) used to prevent the transmission of mtDNA-based disorders. If only a few mitochondria with defective mtDNA are left in the embryo and undergo extensive replication, it might therefore thwart the purpose of MRT. In order to improve the safety and efficacy of this experimental therapy, we need a better understanding of how and which mtDNA is tagged for replication versus transcription after fertilization of the oocyte.

    Original languageEnglish (US)
    JournalEMBO Journal
    DOIs
    Publication statusAccepted/In press - 2017

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    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Immunology and Microbiology(all)
    • Biochemistry, Genetics and Molecular Biology(all)

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