Abstract
Objective: To test the hypothesis that mitochondrial DNA (mtDNA) deletions are more prevalent in granulosa cells from women of advanced reproductive age than from younger women undergoing IVF. Design: Granulosa cells screened for presence or absence of the 4977-bp deletion in human mtDNA. Setting: University-based fertility clinic. Patient(s): Twenty-four women divided equally between two groups: ≤34 years old and ≥38 years old. Intervention(s): Patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration. Main Outcome Measure(s): Presence or absence of the 4977-bp deletion in human mtDNA. Result(s): Seven out of 12 women analyzed who were <38 years old and 0 out of 12 women who were >38 years old had normal mtDNA as indicated by the presence of the 4977-bp fragment. Conclusion(s): These data suggest that women over the age of 38 have granulosa cells that contain a substantial decrease in the level of normal mitochondria as compared with women ≤34 years.
Original language | English (US) |
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Pages (from-to) | 1046-1048 |
Number of pages | 3 |
Journal | Fertility and sterility |
Volume | 78 |
Issue number | 5 |
DOIs | |
State | Published - Nov 1 2002 |
Externally published | Yes |
Keywords
- Apoptosis
- Granulosa cell
- Mitochondria DNA
- Ovarian aging
- Reproductive aging
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology