miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma

Shyamal D. Weeraratne, Vladimir Amani, Adrianne Neiss, Natalia Teider, Deborah K. Scott, Scott L. Pomeroy, Yoon-Jae Cho

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Recent studies have established miR-34a as a key effector of the p53 signaling pathway and have implicated its role in multiple cancer types. Here, we establish that miR-34a induces apoptosis, G2 arrest, and senescence in medulloblastoma and renders these cells more sensitive to chemotherapeutic agents. These effects are mediated in part by the direct post-transcriptional repression of the oncogenic MAGE-A gene family. We demonstrate that miR-34a directly targets the 3' untranslated regions of MAGE-A genes and decreases MAGE-A protein levels. This decrease in MAGE-A results in a concomitant increase in p53 and its associated transcriptional targets, p21/WAF1/CIP1 and, importantly, miR-34a. This establishes a positive feedback mechanism where miR-34a is not only induced by p53 but increases p53 mRNA and protein levels through the modulation of MAGE-A genes. Additionally, the forced expression of miR-34a or the knockdown of MAGE-A genes by small interfering RNA similarly sensitizes medulloblastoma cells to several classes of chemotherapeutic agents, including mitomycin C and cisplatin. Finally, the analysis of mRNA and micro-RNA transcriptional profiles of a series of primary medulloblastomas identifies a subset of tumors with low miR-34a expression and correspondingly high MAGE-A expression, suggesting the coordinate regulation of these genes. Our work establishes a role for miR-34a in modulating responsiveness to chemotherapy in medulloblastoma and presents a novel positive feedback mechanism involving miR-34a and p53, via direct targeting of MAGE-A.

Original languageEnglish (US)
Pages (from-to)165-175
Number of pages11
JournalNeuro-Oncology
Volume13
Issue number2
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

Fingerprint

Medulloblastoma
vif Genes
Messenger RNA
3' Untranslated Regions
Mitomycin
MicroRNAs
Small Interfering RNA
Cisplatin
Genes
Neoplasms
Proteins
Apoptosis
Drug Therapy

Keywords

  • Chemosensitivity
  • MAGE-A
  • Medulloblastoma
  • miR-34a
  • p53
  • Positive feedback mechanism

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Weeraratne, S. D., Amani, V., Neiss, A., Teider, N., Scott, D. K., Pomeroy, S. L., & Cho, Y-J. (2011). miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma. Neuro-Oncology, 13(2), 165-175. https://doi.org/10.1093/neuonc/noq179

miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma. / Weeraratne, Shyamal D.; Amani, Vladimir; Neiss, Adrianne; Teider, Natalia; Scott, Deborah K.; Pomeroy, Scott L.; Cho, Yoon-Jae.

In: Neuro-Oncology, Vol. 13, No. 2, 01.02.2011, p. 165-175.

Research output: Contribution to journalArticle

Weeraratne, SD, Amani, V, Neiss, A, Teider, N, Scott, DK, Pomeroy, SL & Cho, Y-J 2011, 'miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma', Neuro-Oncology, vol. 13, no. 2, pp. 165-175. https://doi.org/10.1093/neuonc/noq179
Weeraratne SD, Amani V, Neiss A, Teider N, Scott DK, Pomeroy SL et al. miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma. Neuro-Oncology. 2011 Feb 1;13(2):165-175. https://doi.org/10.1093/neuonc/noq179
Weeraratne, Shyamal D. ; Amani, Vladimir ; Neiss, Adrianne ; Teider, Natalia ; Scott, Deborah K. ; Pomeroy, Scott L. ; Cho, Yoon-Jae. / miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma. In: Neuro-Oncology. 2011 ; Vol. 13, No. 2. pp. 165-175.
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