miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

Lucia Mendoza-Viveros; Cheng Kang Chiang; Jonathan L.K. Ong; Sara Hegazi; Arthur H. Cheng; Pascale Bouchard-Cannon; Michael Fana; Christopher Lowden; Peng Zhang; Béatrice Bothorel; Matthew G. Michniewicz; Stephen T. Magill; Melissa M. Holmes; Richard H. Goodman; Valérie Simonneaux; Daniel Figeys; Hai Ying M. Cheng

doi:10.1016/j.celrep.2017.03.057

miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

Lucia Mendoza-Viveros, Cheng Kang Chiang, Jonathan L.K. Ong, Sara Hegazi, Arthur H. Cheng, Pascale Bouchard-Cannon, Michael Fana, Christopher Lowden, Peng Zhang, Béatrice Bothorel, Matthew G. Michniewicz, Stephen T. Magill, Melissa M. Holmes, Richard H. Goodman, Valérie Simonneaux, Daniel Figeys, Hai Ying M. Cheng

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

Original language	English (US)
Pages (from-to)	505-520
Number of pages	16
Journal	Cell Reports
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2017.03.057
State	Published - Apr 18 2017

Keywords

MeCP2
circadian rhythms
dendritic morphology
entrainment
miR-132/212
microRNA
seasonal timekeeping
spinogenesis
structural plasticity
suprachiasmatic nucleus

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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Mendoza-Viveros, L., Chiang, C. K., Ong, J. L. K., Hegazi, S., Cheng, A. H., Bouchard-Cannon, P., Fana, M., Lowden, C., Zhang, P., Bothorel, B., Michniewicz, M. G., Magill, S. T., Holmes, M. M., Goodman, R. H., Simonneaux, V., Figeys, D., & Cheng, H. Y. M. (2017). miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock. Cell Reports, 19(3), 505-520. https://doi.org/10.1016/j.celrep.2017.03.057

Mendoza-Viveros, L, Chiang, CK, Ong, JLK, Hegazi, S, Cheng, AH, Bouchard-Cannon, P, Fana, M, Lowden, C, Zhang, P, Bothorel, B, Michniewicz, MG, Magill, ST, Holmes, MM, Goodman, RH, Simonneaux, V, Figeys, D & Cheng, HYM 2017, 'miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock', Cell Reports, vol. 19, no. 3, pp. 505-520. https://doi.org/10.1016/j.celrep.2017.03.057

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abstract = "The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.",

keywords = "MeCP2, circadian rhythms, dendritic morphology, entrainment, miR-132/212, microRNA, seasonal timekeeping, spinogenesis, structural plasticity, suprachiasmatic nucleus",

author = "Lucia Mendoza-Viveros and Chiang, {Cheng Kang} and Ong, {Jonathan L.K.} and Sara Hegazi and Cheng, {Arthur H.} and Pascale Bouchard-Cannon and Michael Fana and Christopher Lowden and Peng Zhang and B{\'e}atrice Bothorel and Michniewicz, {Matthew G.} and Magill, {Stephen T.} and Holmes, {Melissa M.} and Goodman, {Richard H.} and Val{\'e}rie Simonneaux and Daniel Figeys and Cheng, {Hai Ying M.}",

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language = "English (US)",

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T1 - miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

AU - Mendoza-Viveros, Lucia

AU - Chiang, Cheng Kang

AU - Ong, Jonathan L.K.

AU - Hegazi, Sara

AU - Cheng, Arthur H.

AU - Bouchard-Cannon, Pascale

AU - Fana, Michael

AU - Lowden, Christopher

AU - Zhang, Peng

AU - Bothorel, Béatrice

AU - Michniewicz, Matthew G.

AU - Magill, Stephen T.

AU - Holmes, Melissa M.

AU - Goodman, Richard H.

AU - Simonneaux, Valérie

AU - Figeys, Daniel

AU - Cheng, Hai Ying M.

PY - 2017/4/18

Y1 - 2017/4/18

N2 - The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

AB - The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

KW - MeCP2

KW - circadian rhythms

KW - dendritic morphology

KW - entrainment

KW - miR-132/212

KW - microRNA

KW - seasonal timekeeping

KW - spinogenesis

KW - structural plasticity

KW - suprachiasmatic nucleus

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JO - Cell Reports

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ER -

miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

Abstract

Keywords

ASJC Scopus subject areas

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