miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

Lucia Mendoza-Viveros, Cheng Kang Chiang, Jonathan L.K. Ong, Sara Hegazi, Arthur H. Cheng, Pascale Bouchard-Cannon, Michael Fana, Christopher Lowden, Peng Zhang, Béatrice Bothorel, Matthew G. Michniewicz, Stephen T. Magill, Melissa M. Holmes, Richard H. Goodman, Valérie Simonneaux, Daniel Figeys, Hai Ying M. Cheng

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

Original languageEnglish (US)
Pages (from-to)505-520
Number of pages16
JournalCell Reports
Volume19
Issue number3
DOIs
StatePublished - Apr 18 2017

Keywords

  • MeCP2
  • circadian rhythms
  • dendritic morphology
  • entrainment
  • miR-132/212
  • microRNA
  • seasonal timekeeping
  • spinogenesis
  • structural plasticity
  • suprachiasmatic nucleus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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