miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

Lucia Mendoza-Viveros, Cheng Kang Chiang, Jonathan L.K. Ong, Sara Hegazi, Arthur H. Cheng, Pascale Bouchard-Cannon, Michael Fana, Christopher Lowden, Peng Zhang, Béatrice Bothorel, Matthew G. Michniewicz, Stephen T. Magill, Melissa M. Holmes, Richard Goodman, Valérie Simonneaux, Daniel Figeys, Hai Ying M. Cheng

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

Original languageEnglish (US)
Pages (from-to)505-520
Number of pages16
JournalCell Reports
Volume19
Issue number3
DOIs
StatePublished - Apr 18 2017

Fingerprint

Circadian Clocks
Suprachiasmatic Nucleus
Neurons
Clocks
Dendritic Spines
Pacemakers
Melatonin
Ablation
Carrier Proteins
Mesocricetus
Rodentia
Spine

Keywords

  • circadian rhythms
  • dendritic morphology
  • entrainment
  • MeCP2
  • microRNA
  • miR-132/212
  • seasonal timekeeping
  • spinogenesis
  • structural plasticity
  • suprachiasmatic nucleus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mendoza-Viveros, L., Chiang, C. K., Ong, J. L. K., Hegazi, S., Cheng, A. H., Bouchard-Cannon, P., ... Cheng, H. Y. M. (2017). miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock. Cell Reports, 19(3), 505-520. https://doi.org/10.1016/j.celrep.2017.03.057

miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock. / Mendoza-Viveros, Lucia; Chiang, Cheng Kang; Ong, Jonathan L.K.; Hegazi, Sara; Cheng, Arthur H.; Bouchard-Cannon, Pascale; Fana, Michael; Lowden, Christopher; Zhang, Peng; Bothorel, Béatrice; Michniewicz, Matthew G.; Magill, Stephen T.; Holmes, Melissa M.; Goodman, Richard; Simonneaux, Valérie; Figeys, Daniel; Cheng, Hai Ying M.

In: Cell Reports, Vol. 19, No. 3, 18.04.2017, p. 505-520.

Research output: Contribution to journalArticle

Mendoza-Viveros, L, Chiang, CK, Ong, JLK, Hegazi, S, Cheng, AH, Bouchard-Cannon, P, Fana, M, Lowden, C, Zhang, P, Bothorel, B, Michniewicz, MG, Magill, ST, Holmes, MM, Goodman, R, Simonneaux, V, Figeys, D & Cheng, HYM 2017, 'miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock', Cell Reports, vol. 19, no. 3, pp. 505-520. https://doi.org/10.1016/j.celrep.2017.03.057
Mendoza-Viveros L, Chiang CK, Ong JLK, Hegazi S, Cheng AH, Bouchard-Cannon P et al. miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock. Cell Reports. 2017 Apr 18;19(3):505-520. https://doi.org/10.1016/j.celrep.2017.03.057
Mendoza-Viveros, Lucia ; Chiang, Cheng Kang ; Ong, Jonathan L.K. ; Hegazi, Sara ; Cheng, Arthur H. ; Bouchard-Cannon, Pascale ; Fana, Michael ; Lowden, Christopher ; Zhang, Peng ; Bothorel, Béatrice ; Michniewicz, Matthew G. ; Magill, Stephen T. ; Holmes, Melissa M. ; Goodman, Richard ; Simonneaux, Valérie ; Figeys, Daniel ; Cheng, Hai Ying M. / miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock. In: Cell Reports. 2017 ; Vol. 19, No. 3. pp. 505-520.
@article{ced06c2f9af74f86bfc02f921b0ec8df,
title = "miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock",
abstract = "The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.",
keywords = "circadian rhythms, dendritic morphology, entrainment, MeCP2, microRNA, miR-132/212, seasonal timekeeping, spinogenesis, structural plasticity, suprachiasmatic nucleus",
author = "Lucia Mendoza-Viveros and Chiang, {Cheng Kang} and Ong, {Jonathan L.K.} and Sara Hegazi and Cheng, {Arthur H.} and Pascale Bouchard-Cannon and Michael Fana and Christopher Lowden and Peng Zhang and B{\'e}atrice Bothorel and Michniewicz, {Matthew G.} and Magill, {Stephen T.} and Holmes, {Melissa M.} and Richard Goodman and Val{\'e}rie Simonneaux and Daniel Figeys and Cheng, {Hai Ying M.}",
year = "2017",
month = "4",
day = "18",
doi = "10.1016/j.celrep.2017.03.057",
language = "English (US)",
volume = "19",
pages = "505--520",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - miR-132/212 Modulates Seasonal Adaptation and Dendritic Morphology of the Central Circadian Clock

AU - Mendoza-Viveros, Lucia

AU - Chiang, Cheng Kang

AU - Ong, Jonathan L.K.

AU - Hegazi, Sara

AU - Cheng, Arthur H.

AU - Bouchard-Cannon, Pascale

AU - Fana, Michael

AU - Lowden, Christopher

AU - Zhang, Peng

AU - Bothorel, Béatrice

AU - Michniewicz, Matthew G.

AU - Magill, Stephen T.

AU - Holmes, Melissa M.

AU - Goodman, Richard

AU - Simonneaux, Valérie

AU - Figeys, Daniel

AU - Cheng, Hai Ying M.

PY - 2017/4/18

Y1 - 2017/4/18

N2 - The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

AB - The central circadian pacemaker, the suprachiasmatic nucleus (SCN), encodes day length information by mechanisms that are not well understood. Here, we report that genetic ablation of miR-132/212 alters entrainment to different day lengths and non-24 hr day-night cycles, as well as photoperiodic regulation of Period2 expression in the SCN. SCN neurons from miR-132/212-deficient mice have significantly reduced dendritic spine density, along with altered methyl CpG-binding protein (MeCP2) rhythms. In Syrian hamsters, a model seasonal rodent, day length regulates spine density on SCN neurons in a melatonin-independent manner, as well as expression of miR-132, miR-212, and their direct target, MeCP2. Genetic disruption of Mecp2 fully restores the level of dendritic spines of miR-132/212-deficient SCN neurons. Our results reveal that, by regulating the dendritic structure of SCN neurons through a MeCP2-dependent mechanism, miR-132/212 affects the capacity of the SCN to encode seasonal time.

KW - circadian rhythms

KW - dendritic morphology

KW - entrainment

KW - MeCP2

KW - microRNA

KW - miR-132/212

KW - seasonal timekeeping

KW - spinogenesis

KW - structural plasticity

KW - suprachiasmatic nucleus

UR - http://www.scopus.com/inward/record.url?scp=85017590396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017590396&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2017.03.057

DO - 10.1016/j.celrep.2017.03.057

M3 - Article

C2 - 28423315

AN - SCOPUS:85017590396

VL - 19

SP - 505

EP - 520

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 3

ER -