Minimal regulation of platelet activity by PECAM-1

Tarvinder S. Dhanjal, Ewan A. Ross, Jocelyn M. Auger, Owen McCarty, Craig E. Hughes, Yotis A. Senis, Steve P. Watson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

PECAM-1 is a member of the superfamily of immunoglobulins (Ig) and is expressed on platelets at moderate level. PECAM-1 has been reported to have contrasting effects on platelet activation by the collagen receptor GPVI and the integrin, αIIbβ3, even though both receptors signal through Src-kinase regulation of PLCγ2. The present study compares the role of PECAM-1 on platelet activation by these two receptors and by the lectin receptor, CLEC-2, which also signals via PLCγ2. Studies using PECAM-1 knockout-mice and cross-linking of PECAM-1 using specific antibodies demonstrated a minor inhibitory role on platelet responses to the above three receptors and also under some conditions to the G-protein agonist thrombin. The degree of inhibition was considerably less than that produced by PGI2, which elevates cAMP. There was no significant difference in thrombus formation on collagen in PECAM-1-/- platelets relative to litter-matched controls. The very weak inhibitory effect of PECAM-1 on platelet activation relative to that of PGI2 indicate that the Ig-receptor is not a major regulator of platelet activation. PECAM-1 has been reported to have contrasting effects on platelet activation. The present study demonstrates a very mild or negligible effect on platelet activation in response to stimulation by a variety of agonists, thereby questioning the physiological role of the immunoglobulin receptor as a major regulator of platelet activation.

Original languageEnglish (US)
Pages (from-to)56-67
Number of pages12
JournalPlatelets
Volume18
Issue number1
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

CD31 Antigens
Platelet Activation
Blood Platelets
Immunoglobulins
Epoprostenol
Mitogen Receptors
src-Family Kinases
GTP-Binding Proteins
Knockout Mice
Integrins
Thrombin
Thrombosis
Collagen
Antibodies

Keywords

  • CLEC-2
  • GPVI
  • Integrin αβ
  • PECAM-1
  • Platelet activation

ASJC Scopus subject areas

  • Hematology
  • Cell Biology

Cite this

Dhanjal, T. S., Ross, E. A., Auger, J. M., McCarty, O., Hughes, C. E., Senis, Y. A., & Watson, S. P. (2007). Minimal regulation of platelet activity by PECAM-1. Platelets, 18(1), 56-67. https://doi.org/10.1080/09537100600881396

Minimal regulation of platelet activity by PECAM-1. / Dhanjal, Tarvinder S.; Ross, Ewan A.; Auger, Jocelyn M.; McCarty, Owen; Hughes, Craig E.; Senis, Yotis A.; Watson, Steve P.

In: Platelets, Vol. 18, No. 1, 02.2007, p. 56-67.

Research output: Contribution to journalArticle

Dhanjal, TS, Ross, EA, Auger, JM, McCarty, O, Hughes, CE, Senis, YA & Watson, SP 2007, 'Minimal regulation of platelet activity by PECAM-1', Platelets, vol. 18, no. 1, pp. 56-67. https://doi.org/10.1080/09537100600881396
Dhanjal TS, Ross EA, Auger JM, McCarty O, Hughes CE, Senis YA et al. Minimal regulation of platelet activity by PECAM-1. Platelets. 2007 Feb;18(1):56-67. https://doi.org/10.1080/09537100600881396
Dhanjal, Tarvinder S. ; Ross, Ewan A. ; Auger, Jocelyn M. ; McCarty, Owen ; Hughes, Craig E. ; Senis, Yotis A. ; Watson, Steve P. / Minimal regulation of platelet activity by PECAM-1. In: Platelets. 2007 ; Vol. 18, No. 1. pp. 56-67.
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