Midgestational sciatic nerve transection in fetal sheep results in absent nerve regeneration and neurogenic muscle atrophy

Claudia Meuli-Simmen, Martin Meuli, Charles D. Yingling, Thomas Eirnan, Gregory Timmel, Harry J. Buncke, William Lineaweaver, Michael R. Harrison, N. Scott Adzick

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In order to test whether fetal nerve healing and regeneration result in complete functional recovery, we transected the sciatic nerve at trunk level in 13 midgestational sheep fetuses. In 10 fetuses immediate microsurgical nerve coaptation was performed. The neonatal lambs were evaluated clinically, electrophysiologically, and histologically. On the transected side, the 10 surviving lambs showed a sensorimotor sciatic nerve paralysis and atrophy of the muscles innervated by the sciatic nerve. Somatosensory evoked potentials were weakly present in 5 animals and absent in 5 animals. Histologically, minimal signs of axonal regeneration, massive degeneration of the entire nerve, and a marked neurogenic muscle atrophy were found. These unexpected results differ from the findings after peripheral nerve transections in late gestational sheep fetuses and also from the classic wallerian degeneration- regeneration pattern that follows adult nerve injury. We speculate that the almost absent regenerative potential at midgestation is related to axotomy- induced neurotrophic factor deprivation during a developmental phase where the neurons are critically dependent on growth factor for survival.

Original languageEnglish (US)
Pages (from-to)486-492
Number of pages7
JournalPlastic and Reconstructive Surgery
Volume99
Issue number2
DOIs
StatePublished - Feb 1997
Externally publishedYes

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Nerve Regeneration
Muscular Atrophy
Sciatic Nerve
Sheep
Fetus
Regeneration
Wallerian Degeneration
Axotomy
Nerve Degeneration
Somatosensory Evoked Potentials
Nerve Growth Factors
Peripheral Nerves
Paralysis
Intercellular Signaling Peptides and Proteins
Neurons
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Midgestational sciatic nerve transection in fetal sheep results in absent nerve regeneration and neurogenic muscle atrophy. / Meuli-Simmen, Claudia; Meuli, Martin; Yingling, Charles D.; Eirnan, Thomas; Timmel, Gregory; Buncke, Harry J.; Lineaweaver, William; Harrison, Michael R.; Scott Adzick, N.

In: Plastic and Reconstructive Surgery, Vol. 99, No. 2, 02.1997, p. 486-492.

Research output: Contribution to journalArticle

Meuli-Simmen, C, Meuli, M, Yingling, CD, Eirnan, T, Timmel, G, Buncke, HJ, Lineaweaver, W, Harrison, MR & Scott Adzick, N 1997, 'Midgestational sciatic nerve transection in fetal sheep results in absent nerve regeneration and neurogenic muscle atrophy', Plastic and Reconstructive Surgery, vol. 99, no. 2, pp. 486-492. https://doi.org/10.1097/00006534-199702000-00026
Meuli-Simmen, Claudia ; Meuli, Martin ; Yingling, Charles D. ; Eirnan, Thomas ; Timmel, Gregory ; Buncke, Harry J. ; Lineaweaver, William ; Harrison, Michael R. ; Scott Adzick, N. / Midgestational sciatic nerve transection in fetal sheep results in absent nerve regeneration and neurogenic muscle atrophy. In: Plastic and Reconstructive Surgery. 1997 ; Vol. 99, No. 2. pp. 486-492.
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