Mid-molecule parathyroid hormone assay comparison

W. Michael Ivie, Eric S. Orwoll, Michael R. McClung, Terry A. Kenny, J. Robert Swanson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Parathyroid hormone (PTH) assays are becoming available to clinical chemistry laboratories for in-house use. We evaluated the clinical utility of two mid-molecule PTH assays in comparison to a sensitive but technically demanding component assay. A normal reference population, and an abnormal population including cases of primary and secondary hyperparathyroidism, hypoparathyroidism, and humoral hypercalcemia of malignancy were tested. PTH determinations with each assay system and total serum calcium determinations were done on all specimens. The Endocrine Metabolic Center (EMC) kit assay and the Immuno Nuclear Corp. (INC) kit assay demonstrated adequate standard curve reproducibility and precision for clinical use. With the aid of calcium determinations each assay performed well in the separation of abnormal patient groups, and in the separation of abnormal from normal PTH-calcium homeostasis. PTH values for the reference population differed among the three assays. The component assay and the EMC assay had a negative correlation with serum calcium, but the INC assay had a positive correlation with serum calcium.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalClinical Biochemistry
Issue number1
StatePublished - Feb 1986


  • calcium
  • hypercalcemia
  • hyperparathyroidism
  • hyperparathyroidism secondary
  • hypoparathyroidism
  • parathyroid hormones

ASJC Scopus subject areas

  • Clinical Biochemistry


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