Mid-gestation ovine cardiomyocytes are vulnerable to mitotic suppression by thyroid hormone

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Circulating fetal 3,3′,5-tri-iodo-l-thyronine (T3) is maintained at very low levels until a dramatic prepartum surge. 3,3′,5-Tri-iodo-l- thyronine inhibits serum-stimulated proliferation in near-term ovine cardiomyocytes, but it is not known whether midgestation myocytes are also inhibited. Because early cessation of cardiomyocyte mitosis would result in an underendowed heart, we hypothesized that 0.67 gestation (100 of 145 days gestation) ovine cardiomyocytes would be insensitive to suppressive growth effects of T3. These younger cardiomyocytes were grown with T3 in 10% serum-enriched media for 24 hours. Physiological (0.37, 0.75, and 1.5 nmol/L) concentrations of T3 dramatically suppressed mitotic activity in cardiomyocytes (P

Original languageEnglish (US)
Pages (from-to)642-649
Number of pages8
JournalReproductive Sciences
Volume19
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

Thyroid Hormones
Cardiac Myocytes
Sheep
Pregnancy
Thyronines
Serum
Mitosis
Muscle Cells
Growth

Keywords

  • cardiomyocyte proliferation
  • cyclin D1
  • fetal heart
  • p21
  • thyroid hormone

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

@article{ff6b672d2f0943fa9d02b806cd0dd6dc,
title = "Mid-gestation ovine cardiomyocytes are vulnerable to mitotic suppression by thyroid hormone",
abstract = "Circulating fetal 3,3′,5-tri-iodo-l-thyronine (T3) is maintained at very low levels until a dramatic prepartum surge. 3,3′,5-Tri-iodo-l- thyronine inhibits serum-stimulated proliferation in near-term ovine cardiomyocytes, but it is not known whether midgestation myocytes are also inhibited. Because early cessation of cardiomyocyte mitosis would result in an underendowed heart, we hypothesized that 0.67 gestation (100 of 145 days gestation) ovine cardiomyocytes would be insensitive to suppressive growth effects of T3. These younger cardiomyocytes were grown with T3 in 10{\%} serum-enriched media for 24 hours. Physiological (0.37, 0.75, and 1.5 nmol/L) concentrations of T3 dramatically suppressed mitotic activity in cardiomyocytes (P",
keywords = "cardiomyocyte proliferation, cyclin D1, fetal heart, p21, thyroid hormone",
author = "Natasha Chattergoon and Samantha Louey and Philip Stork and George Giraud and Kent Thornburg",
year = "2012",
month = "6",
doi = "10.1177/1933719111432860",
language = "English (US)",
volume = "19",
pages = "642--649",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "6",

}

TY - JOUR

T1 - Mid-gestation ovine cardiomyocytes are vulnerable to mitotic suppression by thyroid hormone

AU - Chattergoon, Natasha

AU - Louey, Samantha

AU - Stork, Philip

AU - Giraud, George

AU - Thornburg, Kent

PY - 2012/6

Y1 - 2012/6

N2 - Circulating fetal 3,3′,5-tri-iodo-l-thyronine (T3) is maintained at very low levels until a dramatic prepartum surge. 3,3′,5-Tri-iodo-l- thyronine inhibits serum-stimulated proliferation in near-term ovine cardiomyocytes, but it is not known whether midgestation myocytes are also inhibited. Because early cessation of cardiomyocyte mitosis would result in an underendowed heart, we hypothesized that 0.67 gestation (100 of 145 days gestation) ovine cardiomyocytes would be insensitive to suppressive growth effects of T3. These younger cardiomyocytes were grown with T3 in 10% serum-enriched media for 24 hours. Physiological (0.37, 0.75, and 1.5 nmol/L) concentrations of T3 dramatically suppressed mitotic activity in cardiomyocytes (P

AB - Circulating fetal 3,3′,5-tri-iodo-l-thyronine (T3) is maintained at very low levels until a dramatic prepartum surge. 3,3′,5-Tri-iodo-l- thyronine inhibits serum-stimulated proliferation in near-term ovine cardiomyocytes, but it is not known whether midgestation myocytes are also inhibited. Because early cessation of cardiomyocyte mitosis would result in an underendowed heart, we hypothesized that 0.67 gestation (100 of 145 days gestation) ovine cardiomyocytes would be insensitive to suppressive growth effects of T3. These younger cardiomyocytes were grown with T3 in 10% serum-enriched media for 24 hours. Physiological (0.37, 0.75, and 1.5 nmol/L) concentrations of T3 dramatically suppressed mitotic activity in cardiomyocytes (P

KW - cardiomyocyte proliferation

KW - cyclin D1

KW - fetal heart

KW - p21

KW - thyroid hormone

UR - http://www.scopus.com/inward/record.url?scp=84862892280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862892280&partnerID=8YFLogxK

U2 - 10.1177/1933719111432860

DO - 10.1177/1933719111432860

M3 - Article

VL - 19

SP - 642

EP - 649

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 6

ER -