Microvascular rheology of definity microbubbles after intra-arterial and intravenous administration

Jonathan Lindner, Ji Song, Ananda R. Jayaweera, Jiri Sklenar, Sanjiv Kaul

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

The microvascular rheology and extent of pulmonary retention of second-generation microbubble ultrasound contrast agents has not previously been well characterized. We assessed the microvascular behavior of Definity, a lipid-shelled microbubble agent containing perfluoropropane gas, using intravital microscopy of either rat spinotrapezius muscle or mouse cremaster muscle. Immediately after intra-arterial injection, which was performed to model pulmonary retention, larger microbubbles (> 5 μm) were entrapped within small arterioles and capillaries. The retention fraction of microbubbles was low (1.2% ± 0.1%) and entrapment was transient (85% dislodged by 10 minutes), resulting in no adverse hemodynamic effects. Leukocyte or platelet adhesion at the site of entrapment was not seen. After intravenous injection, no microbubble entrapment was observed and the velocities of microbubbles in arterioles, venules, and capillaries correlated well with those of red blood cells. We conclude that after intravenous injection and pulmonary passage, the microvascular rheology of Definity microbubbles is similar to that of red blood cells. Microbubble entrapment within the pulmonary microcirculation after venous injection should be negligible and transient. These findings are important for establishing the safety of this agent.

Original languageEnglish (US)
Pages (from-to)396-403
Number of pages8
JournalJournal of the American Society of Echocardiography
Volume15
Issue number5
DOIs
StatePublished - May 2002
Externally publishedYes

Fingerprint

Microbubbles
Rheology
Intravenous Administration
Lung
perflutren
Arterioles
Intravenous Injections
Erythrocytes
Intra-Arterial Injections
Abdominal Muscles
Venules
Definity
Microcirculation
Contrast Media
Leukocytes
Blood Platelets
Gases
Hemodynamics
Lipids
Safety

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Microvascular rheology of definity microbubbles after intra-arterial and intravenous administration. / Lindner, Jonathan; Song, Ji; Jayaweera, Ananda R.; Sklenar, Jiri; Kaul, Sanjiv.

In: Journal of the American Society of Echocardiography, Vol. 15, No. 5, 05.2002, p. 396-403.

Research output: Contribution to journalArticle

@article{a355b4e47e9940babdec7819634c94ff,
title = "Microvascular rheology of definity microbubbles after intra-arterial and intravenous administration",
abstract = "The microvascular rheology and extent of pulmonary retention of second-generation microbubble ultrasound contrast agents has not previously been well characterized. We assessed the microvascular behavior of Definity, a lipid-shelled microbubble agent containing perfluoropropane gas, using intravital microscopy of either rat spinotrapezius muscle or mouse cremaster muscle. Immediately after intra-arterial injection, which was performed to model pulmonary retention, larger microbubbles (> 5 μm) were entrapped within small arterioles and capillaries. The retention fraction of microbubbles was low (1.2{\%} ± 0.1{\%}) and entrapment was transient (85{\%} dislodged by 10 minutes), resulting in no adverse hemodynamic effects. Leukocyte or platelet adhesion at the site of entrapment was not seen. After intravenous injection, no microbubble entrapment was observed and the velocities of microbubbles in arterioles, venules, and capillaries correlated well with those of red blood cells. We conclude that after intravenous injection and pulmonary passage, the microvascular rheology of Definity microbubbles is similar to that of red blood cells. Microbubble entrapment within the pulmonary microcirculation after venous injection should be negligible and transient. These findings are important for establishing the safety of this agent.",
author = "Jonathan Lindner and Ji Song and Jayaweera, {Ananda R.} and Jiri Sklenar and Sanjiv Kaul",
year = "2002",
month = "5",
doi = "10.1067/mje.2002.117290",
language = "English (US)",
volume = "15",
pages = "396--403",
journal = "Journal of the American Society of Echocardiography",
issn = "0894-7317",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Microvascular rheology of definity microbubbles after intra-arterial and intravenous administration

AU - Lindner, Jonathan

AU - Song, Ji

AU - Jayaweera, Ananda R.

AU - Sklenar, Jiri

AU - Kaul, Sanjiv

PY - 2002/5

Y1 - 2002/5

N2 - The microvascular rheology and extent of pulmonary retention of second-generation microbubble ultrasound contrast agents has not previously been well characterized. We assessed the microvascular behavior of Definity, a lipid-shelled microbubble agent containing perfluoropropane gas, using intravital microscopy of either rat spinotrapezius muscle or mouse cremaster muscle. Immediately after intra-arterial injection, which was performed to model pulmonary retention, larger microbubbles (> 5 μm) were entrapped within small arterioles and capillaries. The retention fraction of microbubbles was low (1.2% ± 0.1%) and entrapment was transient (85% dislodged by 10 minutes), resulting in no adverse hemodynamic effects. Leukocyte or platelet adhesion at the site of entrapment was not seen. After intravenous injection, no microbubble entrapment was observed and the velocities of microbubbles in arterioles, venules, and capillaries correlated well with those of red blood cells. We conclude that after intravenous injection and pulmonary passage, the microvascular rheology of Definity microbubbles is similar to that of red blood cells. Microbubble entrapment within the pulmonary microcirculation after venous injection should be negligible and transient. These findings are important for establishing the safety of this agent.

AB - The microvascular rheology and extent of pulmonary retention of second-generation microbubble ultrasound contrast agents has not previously been well characterized. We assessed the microvascular behavior of Definity, a lipid-shelled microbubble agent containing perfluoropropane gas, using intravital microscopy of either rat spinotrapezius muscle or mouse cremaster muscle. Immediately after intra-arterial injection, which was performed to model pulmonary retention, larger microbubbles (> 5 μm) were entrapped within small arterioles and capillaries. The retention fraction of microbubbles was low (1.2% ± 0.1%) and entrapment was transient (85% dislodged by 10 minutes), resulting in no adverse hemodynamic effects. Leukocyte or platelet adhesion at the site of entrapment was not seen. After intravenous injection, no microbubble entrapment was observed and the velocities of microbubbles in arterioles, venules, and capillaries correlated well with those of red blood cells. We conclude that after intravenous injection and pulmonary passage, the microvascular rheology of Definity microbubbles is similar to that of red blood cells. Microbubble entrapment within the pulmonary microcirculation after venous injection should be negligible and transient. These findings are important for establishing the safety of this agent.

UR - http://www.scopus.com/inward/record.url?scp=0036582627&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036582627&partnerID=8YFLogxK

U2 - 10.1067/mje.2002.117290

DO - 10.1067/mje.2002.117290

M3 - Article

C2 - 12019422

AN - SCOPUS:0036582627

VL - 15

SP - 396

EP - 403

JO - Journal of the American Society of Echocardiography

JF - Journal of the American Society of Echocardiography

SN - 0894-7317

IS - 5

ER -