Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy

Ravikant Samatham, David Levitz, Reid Fletcher, Yongji Fu, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Preparation of phantoms with reproducible and homogenous optical properties is tricky. The microscopic heterogeneity and macroscopic homogeneity of tissue phantoms were compared using reflectance-mode confocal laser scanning microscopy. Tissue phantoms were prepared using polystyrene microspheres as scattering medium in aqueous and gel matrix. Uniform distribution of microparticles in phantoms was evaluated by confocal imaging. Comparison of the heterogeneity of the phantoms was accomplished based on microscopic optical scattering properties. Distribution of optical properties at the microscopic levels was determined by a simple theory developed based on the depth-dependent decay of the reflectance-mode confocal signal. The variability of these optical properties is correlated to heterogeneity of the phantom. These microscopic properties were compared with macroscopic properties determined by ballistic transmission experiment. This enabled to optimize the phantom preparation procedure.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6870
DOIs
StatePublished - 2008
EventDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue - San Jose, CA, United States
Duration: Jan 19 2008Jan 21 2008

Other

OtherDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
CountryUnited States
CitySan Jose, CA
Period1/19/081/21/08

Fingerprint

Laser modes
Microscopic examination
Optical properties
Tissue
Scanning
Lasers
Scattering
Ballistics
Microspheres
Polystyrenes
Gels
Imaging techniques
Experiments

Keywords

  • Collimated transmission
  • Optical scattering properties
  • Reflectance-mode confocal scanning laser microscope
  • Tissue phantoms

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Samatham, R., Levitz, D., Fletcher, R., Fu, Y., & Jacques, S. (2008). Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6870). [68700H] https://doi.org/10.1117/12.764073

Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy. / Samatham, Ravikant; Levitz, David; Fletcher, Reid; Fu, Yongji; Jacques, Steven.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6870 2008. 68700H.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Samatham, R, Levitz, D, Fletcher, R, Fu, Y & Jacques, S 2008, Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6870, 68700H, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, San Jose, CA, United States, 1/19/08. https://doi.org/10.1117/12.764073
Samatham R, Levitz D, Fletcher R, Fu Y, Jacques S. Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6870. 2008. 68700H https://doi.org/10.1117/12.764073
Samatham, Ravikant ; Levitz, David ; Fletcher, Reid ; Fu, Yongji ; Jacques, Steven. / Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6870 2008.
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