Microscopic computed tomography-based skeletal phenotyping for genetic model organisms

Suresh I. Prajapati, Lisa Nevell, Charles Keller

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Forward and reverse genetics now enable researchers to understand embryonic and postnatal gene functioning in a wide range of species. Some genetic mutations cause obvious morphological change, whereas other mutations can lead to more subtle phenotypes and might be overlooked without adequate observations and quantifications. Due to the increase in number of genetic model organisms examined by the growing field of phenomics, standardized but sensitive methods for quantitative analysis are increasingly necessary in the everyday practice of analyzing ever-increasing quantities of phenotypic data. In this chapter, we have presented platform-independent parameters for the use of microscopic X-ray computed tomography (microCT) for phenotyping species-specific skeletal morphology of a variety of different genetic model organisms.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages221-226
Number of pages6
Volume1092
ISBN (Print)9781603272902
DOIs
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1092
ISSN (Print)10643745

Fingerprint

Genetic Models
Tomography
Reverse Genetics
Mutation
X Ray Computed Tomography
Research Personnel
Phenotype
Genes

Keywords

  • Embryogenesis
  • Genetic model organisms
  • microCT
  • Skeletal phenotyping

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Prajapati, S. I., Nevell, L., & Keller, C. (2014). Microscopic computed tomography-based skeletal phenotyping for genetic model organisms. In Methods in Molecular Biology (Vol. 1092, pp. 221-226). (Methods in Molecular Biology; Vol. 1092). Humana Press Inc.. https://doi.org/10.1007/978-1-60327-292-6_14

Microscopic computed tomography-based skeletal phenotyping for genetic model organisms. / Prajapati, Suresh I.; Nevell, Lisa; Keller, Charles.

Methods in Molecular Biology. Vol. 1092 Humana Press Inc., 2014. p. 221-226 (Methods in Molecular Biology; Vol. 1092).

Research output: Chapter in Book/Report/Conference proceedingChapter

Prajapati, SI, Nevell, L & Keller, C 2014, Microscopic computed tomography-based skeletal phenotyping for genetic model organisms. in Methods in Molecular Biology. vol. 1092, Methods in Molecular Biology, vol. 1092, Humana Press Inc., pp. 221-226. https://doi.org/10.1007/978-1-60327-292-6_14
Prajapati SI, Nevell L, Keller C. Microscopic computed tomography-based skeletal phenotyping for genetic model organisms. In Methods in Molecular Biology. Vol. 1092. Humana Press Inc. 2014. p. 221-226. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-292-6_14
Prajapati, Suresh I. ; Nevell, Lisa ; Keller, Charles. / Microscopic computed tomography-based skeletal phenotyping for genetic model organisms. Methods in Molecular Biology. Vol. 1092 Humana Press Inc., 2014. pp. 221-226 (Methods in Molecular Biology).
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