Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta)

Sreetharan Kanthaswamy, Andrea Von Dollen, Jennifer D. Kurushima, Ona Alminas, Jeffrey Rogers, Betsy Ferguson, Nicholas W. Lerche, Philip C. Allen, David Glenn Smith

    Research output: Contribution to journalArticle

    30 Scopus citations

    Abstract

    To preserve genetic variability and minimize genetic subdivision among captive Macaca, mulatta at each of the U.S. National Institutes of Health (NIH)-sponsored regional research colonies, the genetic structure of each colony must be characterized. To compare population genetic and demographic parameters across colonies and generations, one standard panel of highly informative genetic markers is required. We assembled a core marker set of four multiplex polymerase chain reaction (PCR) panels comprising 15 autosomal short tandem repeat (STR) loci with high information content selected from existing panels of well-characterized markers that are currently used for parentage assessment and genetic management of rhesus macaques. We then assessed the effectiveness of these loci for providing high probabilities of individual identification and parentage resolution, and for estimating population genetic parameters that are useful for genetic management.

    Original languageEnglish (US)
    Pages (from-to)73-95
    Number of pages23
    JournalAmerican Journal of Primatology
    Volume68
    Issue number1
    DOIs
    Publication statusPublished - Jan 2006

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    Keywords

    • Genetic diversity
    • Heterozygosity
    • Polymorphic information content (PIC)
    • Probability of genetic identity (PI)
    • Probability of parentage exclusion (PE)
    • Short tandem repeats (STRs)

    ASJC Scopus subject areas

    • Animal Science and Zoology

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