MicroRNA expression signature of human sarcomas

S. Subramanian, W. O. Lui, C. H. Lee, I. Espinosa, T. O. Nielsen, Michael Heinrich, Christopher Corless, A. Z. Fire, M. Van De Rijn

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are ∼22 nucleotide-long noncoding RNAs involved in several biological processes including development, differentiation and proliferation. Recent studies suggest that knowledge of miRNA expression patterns in cancer may have substantial value for diagnostic and prognostic determinations as well as for eventual therapeutic intervention. We performed comprehensive analysis of miRNA expression profiles of 27 sarcomas, 5 normal smooth muscle and 2 normal skeletal muscle tissues using microarray technology and/or small RNA cloning approaches. The miRNA expression profiles are distinct among the tumor types as demonstrated by an unsupervised hierarchical clustering, and unique miRNA expression signatures were identified in each tumor class. Remarkably, the miRNA expression patterns suggested that two of the sarcomas had been misdiagnosed and this was confirmed by reevaluation of the tumors using histopathologic and molecular analyses. Using the cloning approach, we also identified 31 novel miRNAs or other small RNA effectors in the sarcomas and normal skeletal muscle tissues examined. Our data show that different histological types of sarcoma have distinct miRNA expression patterns, reflecting the apparent lineage and differentiation status of the tumors. The identification of unique miRNA signatures in each tumor type may indicate their role in tumorigenesis and may aid in diagnosis of soft tissue sarcomas.

Original languageEnglish (US)
Pages (from-to)2015-2026
Number of pages12
JournalOncogene
Volume27
Issue number14
DOIs
StatePublished - Mar 27 2008

Fingerprint

MicroRNAs
Sarcoma
Neoplasms
Organism Cloning
Skeletal Muscle
Long Noncoding RNA
RNA
Biological Phenomena
Muscles
Diagnostic Errors
Smooth Muscle
Cluster Analysis
Carcinogenesis
Nucleotides
Technology

Keywords

  • Microarrays
  • miRNA
  • Sarcoma

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Subramanian, S., Lui, W. O., Lee, C. H., Espinosa, I., Nielsen, T. O., Heinrich, M., ... Van De Rijn, M. (2008). MicroRNA expression signature of human sarcomas. Oncogene, 27(14), 2015-2026. https://doi.org/10.1038/sj.onc.1210836

MicroRNA expression signature of human sarcomas. / Subramanian, S.; Lui, W. O.; Lee, C. H.; Espinosa, I.; Nielsen, T. O.; Heinrich, Michael; Corless, Christopher; Fire, A. Z.; Van De Rijn, M.

In: Oncogene, Vol. 27, No. 14, 27.03.2008, p. 2015-2026.

Research output: Contribution to journalArticle

Subramanian, S, Lui, WO, Lee, CH, Espinosa, I, Nielsen, TO, Heinrich, M, Corless, C, Fire, AZ & Van De Rijn, M 2008, 'MicroRNA expression signature of human sarcomas', Oncogene, vol. 27, no. 14, pp. 2015-2026. https://doi.org/10.1038/sj.onc.1210836
Subramanian S, Lui WO, Lee CH, Espinosa I, Nielsen TO, Heinrich M et al. MicroRNA expression signature of human sarcomas. Oncogene. 2008 Mar 27;27(14):2015-2026. https://doi.org/10.1038/sj.onc.1210836
Subramanian, S. ; Lui, W. O. ; Lee, C. H. ; Espinosa, I. ; Nielsen, T. O. ; Heinrich, Michael ; Corless, Christopher ; Fire, A. Z. ; Van De Rijn, M. / MicroRNA expression signature of human sarcomas. In: Oncogene. 2008 ; Vol. 27, No. 14. pp. 2015-2026.
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