MicroRNA expression signature of human sarcomas

S. Subramanian, W. O. Lui, C. H. Lee, I. Espinosa, T. O. Nielsen, M. C. Heinrich, C. L. Corless, A. Z. Fire, M. Van De Rijn

Research output: Contribution to journalArticle

188 Scopus citations

Abstract

MicroRNAs (miRNAs) are ∼22 nucleotide-long noncoding RNAs involved in several biological processes including development, differentiation and proliferation. Recent studies suggest that knowledge of miRNA expression patterns in cancer may have substantial value for diagnostic and prognostic determinations as well as for eventual therapeutic intervention. We performed comprehensive analysis of miRNA expression profiles of 27 sarcomas, 5 normal smooth muscle and 2 normal skeletal muscle tissues using microarray technology and/or small RNA cloning approaches. The miRNA expression profiles are distinct among the tumor types as demonstrated by an unsupervised hierarchical clustering, and unique miRNA expression signatures were identified in each tumor class. Remarkably, the miRNA expression patterns suggested that two of the sarcomas had been misdiagnosed and this was confirmed by reevaluation of the tumors using histopathologic and molecular analyses. Using the cloning approach, we also identified 31 novel miRNAs or other small RNA effectors in the sarcomas and normal skeletal muscle tissues examined. Our data show that different histological types of sarcoma have distinct miRNA expression patterns, reflecting the apparent lineage and differentiation status of the tumors. The identification of unique miRNA signatures in each tumor type may indicate their role in tumorigenesis and may aid in diagnosis of soft tissue sarcomas.

Original languageEnglish (US)
Pages (from-to)2015-2026
Number of pages12
JournalOncogene
Volume27
Issue number14
DOIs
StatePublished - Mar 27 2008

Keywords

  • Microarrays
  • Sarcoma
  • miRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'MicroRNA expression signature of human sarcomas'. Together they form a unique fingerprint.

  • Cite this

    Subramanian, S., Lui, W. O., Lee, C. H., Espinosa, I., Nielsen, T. O., Heinrich, M. C., Corless, C. L., Fire, A. Z., & Van De Rijn, M. (2008). MicroRNA expression signature of human sarcomas. Oncogene, 27(14), 2015-2026. https://doi.org/10.1038/sj.onc.1210836