MicroRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus

Stephen T. Magill, Xiaolu Cambronne, Bryan W. Luikart, Daniel T. Lioy, Barbara H. Leighton, Gary Westbrook, Gail Mandel, Richard Goodman

Research output: Contribution to journalArticle

281 Citations (Scopus)

Abstract

Newborn neurons in the dentate gyrus of the adult hippocampus rely upon cAMP response element binding protein (CREB) signaling for their differentiation into mature granule cells and their integration into the dentate network. Among its many targets, the transcription factor CREB activates expression of a gene locus that produces two microRNAs, miR-132 and miR-212. In cultured cortical and hippocampal neurons, miR-132 functions downstream from CREB to mediate activity-dependent dendritic growth and spine formation in response to a variety of signaling pathways. To investigate whether miR-132 and/or miR-212 contribute to the maturation of dendrites in newborn neurons in the adult hippocampus, we inserted LoxP sites surrounding the miR-212/132 locus and specifically targeted its deletion by stereotactically injecting a retrovirus expressing Cre recombinase. Deletion of the miR- 212/132 locus caused a dramatic decrease in dendrite length, arborization, and spine density. The miR-212/132 locus may express up to four distinct microRNAs, miR-132 and -212 and their reverse strands miR-132* and -212*. Using ratiometric microRNA sensors, we determined that miR-132 is the predominantly active product in hippocampal neurons. We conclude that miR-132 is required for normal dendrite maturation in newborn neurons in the adult hippocampus and suggest that this microRNA also may participate in other examples of CREB-mediated signaling.

Original languageEnglish (US)
Pages (from-to)20382-20387
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number47
DOIs
StatePublished - Nov 23 2010

Fingerprint

Neuronal Plasticity
Cyclic AMP Response Element-Binding Protein
MicroRNAs
Hippocampus
Neurons
Growth
Dendrites
Parahippocampal Gyrus
Dendritic Spines
Dentate Gyrus
Retroviridae
Spine
Transcription Factors
Gene Expression

Keywords

  • Learning
  • MicroRNA-212
  • Neurogenesis
  • Plasticity

ASJC Scopus subject areas

  • General

Cite this

MicroRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus. / Magill, Stephen T.; Cambronne, Xiaolu; Luikart, Bryan W.; Lioy, Daniel T.; Leighton, Barbara H.; Westbrook, Gary; Mandel, Gail; Goodman, Richard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 47, 23.11.2010, p. 20382-20387.

Research output: Contribution to journalArticle

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