Microfluidics and coagulation biology

Thomas V. Colace, Garth W. Tormoen, Owen McCarty, Scott L. Diamond

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The study of blood ex vivo can occur in closed or open systems, with or without flow. Microfluidic devices, which constrain fluids to a small (typically submillimeter) scale, facilitate analysis of platelet function, coagulation biology, cellular biorheology, adhesion dynamics, and pharmacology and, as a result, can be an invaluable tool for clinical diagnostics. An experimental session can accommodate hundreds to thousands of unique clotting, or thrombotic, events. Using microfluidics, thrombotic events can be studied on defined surfaces of biopolymers, matrix proteins, and tissue factor, under constant flow rate or constant pressure drop conditions. Distinct shear rates can be generated on a device using a single perfusion pump. Microfluidics facilitated both the determination of intraluminal thrombus permeability and the discovery that platelet contractility can be activated by a sudden decrease in flow. Microfluidic devices are ideal for multicolor imaging of platelets, fibrin, and phosphatidylserine and provide a human blood analog to mouse injury models. Overall, microfluidic advances offer many opportunities for research, drug testing under relevant hemodynamic conditions, and clinical diagnostics.

Original languageEnglish (US)
Pages (from-to)283-303
Number of pages21
JournalAnnual Review of Biomedical Engineering
Volume15
DOIs
StatePublished - Jul 2013

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Coagulation
Blood Platelets
Platelets
Infusion Pumps
Biopolymers
Phosphatidylserines
Thromboplastin
Fibrin
Blood
Cell Biology
Cytology
Permeability
Thrombosis
Hemodynamics
Pharmacology
Open systems
Pressure
Equipment and Supplies

Keywords

  • collagen
  • fibrin
  • hemostasis
  • platelet function
  • thrombosis
  • von Willebrand factor

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Microfluidics and coagulation biology. / Colace, Thomas V.; Tormoen, Garth W.; McCarty, Owen; Diamond, Scott L.

In: Annual Review of Biomedical Engineering, Vol. 15, 07.2013, p. 283-303.

Research output: Contribution to journalArticle

Colace, Thomas V. ; Tormoen, Garth W. ; McCarty, Owen ; Diamond, Scott L. / Microfluidics and coagulation biology. In: Annual Review of Biomedical Engineering. 2013 ; Vol. 15. pp. 283-303.
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