Microfluidic sample preparation of pleural effusions for cytodiagnostics

Albert J. Mach, Derek E. Go, James Che, Ish Talati, Yong Ying, Rajan Kulkarni, Jianyu Rao, Dino Di Carlo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Pleural effusions - fluids that build up surrounding the lungs - can harbor malignant cells which are important to identify for diagnosis of cancer. However, rare malignant cells may lead to misleading diagnoses and a large background of leukocytes can prevent accurate identification of mutations of interest for targeted anti-cancer therapies. We developed a miniaturized microfluidic system that employs microscale vortices for the size-based isolation of malignant cancer cells and mesothelial cells. By processing mL volumes of pleural effusions and enriching target cells over a background of blood cells, we replace the traditional centrifugation step in the clinical lab. We demonstrate higher purity in 100% of the samples (n=30) where cells are made readily available for immunolabeling, standard cytology analysis and molecular analysis.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages803-805
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Inertial microfluidics
  • Laminar microvortices
  • Liquid-based preparation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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