TY - GEN
T1 - Microfluidic sample preparation of pleural effusions for cytodiagnostics
AU - Mach, Albert J.
AU - Go, Derek E.
AU - Che, James
AU - Talati, Ish
AU - Ying, Yong
AU - Kulkarni, Rajan
AU - Rao, Jianyu
AU - Di Carlo, Dino
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Pleural effusions - fluids that build up surrounding the lungs - can harbor malignant cells which are important to identify for diagnosis of cancer. However, rare malignant cells may lead to misleading diagnoses and a large background of leukocytes can prevent accurate identification of mutations of interest for targeted anti-cancer therapies. We developed a miniaturized microfluidic system that employs microscale vortices for the size-based isolation of malignant cancer cells and mesothelial cells. By processing mL volumes of pleural effusions and enriching target cells over a background of blood cells, we replace the traditional centrifugation step in the clinical lab. We demonstrate higher purity in 100% of the samples (n=30) where cells are made readily available for immunolabeling, standard cytology analysis and molecular analysis.
AB - Pleural effusions - fluids that build up surrounding the lungs - can harbor malignant cells which are important to identify for diagnosis of cancer. However, rare malignant cells may lead to misleading diagnoses and a large background of leukocytes can prevent accurate identification of mutations of interest for targeted anti-cancer therapies. We developed a miniaturized microfluidic system that employs microscale vortices for the size-based isolation of malignant cancer cells and mesothelial cells. By processing mL volumes of pleural effusions and enriching target cells over a background of blood cells, we replace the traditional centrifugation step in the clinical lab. We demonstrate higher purity in 100% of the samples (n=30) where cells are made readily available for immunolabeling, standard cytology analysis and molecular analysis.
KW - Inertial microfluidics
KW - Laminar microvortices
KW - Liquid-based preparation
UR - http://www.scopus.com/inward/record.url?scp=84901753423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901753423&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901753423
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 803
EP - 805
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -