Microfluidic device and culture platform for the observation and control of axonal growth and axonal organelle transport of rat hippocampal neurons

Alexander C. Barbati, Cheng Fang, Gary Banker, Brian J. Kirby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the design, fabrication, and implementation of a device to culture rat hippocampal neurons guided by arrays of cell-adhesive biopolymers on traditional cell culture substrates. These guided arrays of cells are shown to permit live-imaging of mitochondrial transport through the cell axon. We further report the development of a microfluidic device to deliver solutes to discrete sections of rat hippocampal neurons with spatial and temporal resolution.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages481-483
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Microfluidics
Neurons
Rats
Biopolymers
Cell culture
Adhesives
Imaging techniques
Fabrication
Substrates
Axons

Keywords

  • Axonal growth
  • Imaging organelle transport
  • Neural cell culture

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Barbati, A. C., Fang, C., Banker, G., & Kirby, B. J. (2009). Microfluidic device and culture platform for the observation and control of axonal growth and axonal organelle transport of rat hippocampal neurons. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 481-483). Chemical and Biological Microsystems Society.

Microfluidic device and culture platform for the observation and control of axonal growth and axonal organelle transport of rat hippocampal neurons. / Barbati, Alexander C.; Fang, Cheng; Banker, Gary; Kirby, Brian J.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 481-483.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Barbati, AC, Fang, C, Banker, G & Kirby, BJ 2009, Microfluidic device and culture platform for the observation and control of axonal growth and axonal organelle transport of rat hippocampal neurons. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 481-483, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Barbati AC, Fang C, Banker G, Kirby BJ. Microfluidic device and culture platform for the observation and control of axonal growth and axonal organelle transport of rat hippocampal neurons. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 481-483
Barbati, Alexander C. ; Fang, Cheng ; Banker, Gary ; Kirby, Brian J. / Microfluidic device and culture platform for the observation and control of axonal growth and axonal organelle transport of rat hippocampal neurons. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 481-483
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