Microbial characterization of groundwater undergoing treatment with a permeable reactive iron barrier

Marcio L B Da Silva, Richard Johnson, Pedro J J Alvarez

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Phylogenetic analyses of micro-organisms in groundwater samples from within and around a zero-valent iron (ZVI) permeable reactive barrier (PRB) identified several bacteria that could utilize H2 produced during anaerobic ZVI corrosion and residual guar biopolymer used during PRB installation. Some of these bacteria are likely contributing to the removal of some groundwater constituents (i.e., sulfate). Bacteria concentrations increased from ∼101 cells mL-1 at 2 m upgradient to ∼10 2 cells mL-1 within the PRB and ∼104 cells mL-1 at 2 to 6 m downgradient. This trend possibly reflects increased substrate availability through the PRB, although a corrosion-induced increase in pH beyond optimum levels within the iron layer (from pH 7 to 9.8) may have limited microbial colonization. Micro-organisms that were detected using quantitative PCR include (iron reducing) Geobacter sp. (putative methanogenic) Archaea, and (sulfate reducing) δ-proteobacteria such as Desulfuromonadales sp. Sequencing of DGGE bands also revealed the presence of uncultured dissimilatory metal reducers and Clostridia sp., which was dominant in a sample collected within the ZVI-PRB. These results suggest that indigenous microbial communities are likely to experience population shifts when ZVI-PRBs are installed to exploit several metabolic niches that evolve when ZVI corrodes. Whether such population shifts enhance ZVI-PRB performance requires further investigation.

Original languageEnglish (US)
Pages (from-to)1122-1127
Number of pages6
JournalEnvironmental Engineering Science
Volume24
Issue number8
DOIs
StatePublished - Oct 1 2007

Fingerprint

reactive barrier
Groundwater
Iron
iron
groundwater
Bacteria
Sulfates
bacterium
corrosion
Corrosion
sulfate
Clostridium
Biopolymers
microbial community
niche
colonization
Metals
Availability
phylogenetics
substrate

Keywords

  • Groundwater samples
  • Permeable reactive barrier
  • Phylogenetic analyses
  • Zero-valent iron

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Microbial characterization of groundwater undergoing treatment with a permeable reactive iron barrier. / Da Silva, Marcio L B; Johnson, Richard; Alvarez, Pedro J J.

In: Environmental Engineering Science, Vol. 24, No. 8, 01.10.2007, p. 1122-1127.

Research output: Contribution to journalArticle

@article{e10105b529d94356a52a8df879402736,
title = "Microbial characterization of groundwater undergoing treatment with a permeable reactive iron barrier",
abstract = "Phylogenetic analyses of micro-organisms in groundwater samples from within and around a zero-valent iron (ZVI) permeable reactive barrier (PRB) identified several bacteria that could utilize H2 produced during anaerobic ZVI corrosion and residual guar biopolymer used during PRB installation. Some of these bacteria are likely contributing to the removal of some groundwater constituents (i.e., sulfate). Bacteria concentrations increased from ∼101 cells mL-1 at 2 m upgradient to ∼10 2 cells mL-1 within the PRB and ∼104 cells mL-1 at 2 to 6 m downgradient. This trend possibly reflects increased substrate availability through the PRB, although a corrosion-induced increase in pH beyond optimum levels within the iron layer (from pH 7 to 9.8) may have limited microbial colonization. Micro-organisms that were detected using quantitative PCR include (iron reducing) Geobacter sp. (putative methanogenic) Archaea, and (sulfate reducing) δ-proteobacteria such as Desulfuromonadales sp. Sequencing of DGGE bands also revealed the presence of uncultured dissimilatory metal reducers and Clostridia sp., which was dominant in a sample collected within the ZVI-PRB. These results suggest that indigenous microbial communities are likely to experience population shifts when ZVI-PRBs are installed to exploit several metabolic niches that evolve when ZVI corrodes. Whether such population shifts enhance ZVI-PRB performance requires further investigation.",
keywords = "Groundwater samples, Permeable reactive barrier, Phylogenetic analyses, Zero-valent iron",
author = "{Da Silva}, {Marcio L B} and Richard Johnson and Alvarez, {Pedro J J}",
year = "2007",
month = "10",
day = "1",
doi = "10.1089/ees.2007.0016",
language = "English (US)",
volume = "24",
pages = "1122--1127",
journal = "Environmental Engineering Science",
issn = "1092-8758",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

TY - JOUR

T1 - Microbial characterization of groundwater undergoing treatment with a permeable reactive iron barrier

AU - Da Silva, Marcio L B

AU - Johnson, Richard

AU - Alvarez, Pedro J J

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Phylogenetic analyses of micro-organisms in groundwater samples from within and around a zero-valent iron (ZVI) permeable reactive barrier (PRB) identified several bacteria that could utilize H2 produced during anaerobic ZVI corrosion and residual guar biopolymer used during PRB installation. Some of these bacteria are likely contributing to the removal of some groundwater constituents (i.e., sulfate). Bacteria concentrations increased from ∼101 cells mL-1 at 2 m upgradient to ∼10 2 cells mL-1 within the PRB and ∼104 cells mL-1 at 2 to 6 m downgradient. This trend possibly reflects increased substrate availability through the PRB, although a corrosion-induced increase in pH beyond optimum levels within the iron layer (from pH 7 to 9.8) may have limited microbial colonization. Micro-organisms that were detected using quantitative PCR include (iron reducing) Geobacter sp. (putative methanogenic) Archaea, and (sulfate reducing) δ-proteobacteria such as Desulfuromonadales sp. Sequencing of DGGE bands also revealed the presence of uncultured dissimilatory metal reducers and Clostridia sp., which was dominant in a sample collected within the ZVI-PRB. These results suggest that indigenous microbial communities are likely to experience population shifts when ZVI-PRBs are installed to exploit several metabolic niches that evolve when ZVI corrodes. Whether such population shifts enhance ZVI-PRB performance requires further investigation.

AB - Phylogenetic analyses of micro-organisms in groundwater samples from within and around a zero-valent iron (ZVI) permeable reactive barrier (PRB) identified several bacteria that could utilize H2 produced during anaerobic ZVI corrosion and residual guar biopolymer used during PRB installation. Some of these bacteria are likely contributing to the removal of some groundwater constituents (i.e., sulfate). Bacteria concentrations increased from ∼101 cells mL-1 at 2 m upgradient to ∼10 2 cells mL-1 within the PRB and ∼104 cells mL-1 at 2 to 6 m downgradient. This trend possibly reflects increased substrate availability through the PRB, although a corrosion-induced increase in pH beyond optimum levels within the iron layer (from pH 7 to 9.8) may have limited microbial colonization. Micro-organisms that were detected using quantitative PCR include (iron reducing) Geobacter sp. (putative methanogenic) Archaea, and (sulfate reducing) δ-proteobacteria such as Desulfuromonadales sp. Sequencing of DGGE bands also revealed the presence of uncultured dissimilatory metal reducers and Clostridia sp., which was dominant in a sample collected within the ZVI-PRB. These results suggest that indigenous microbial communities are likely to experience population shifts when ZVI-PRBs are installed to exploit several metabolic niches that evolve when ZVI corrodes. Whether such population shifts enhance ZVI-PRB performance requires further investigation.

KW - Groundwater samples

KW - Permeable reactive barrier

KW - Phylogenetic analyses

KW - Zero-valent iron

UR - http://www.scopus.com/inward/record.url?scp=35348965195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35348965195&partnerID=8YFLogxK

U2 - 10.1089/ees.2007.0016

DO - 10.1089/ees.2007.0016

M3 - Article

VL - 24

SP - 1122

EP - 1127

JO - Environmental Engineering Science

JF - Environmental Engineering Science

SN - 1092-8758

IS - 8

ER -