MHYT, a new integral membrane sensor domain

Michael Y. Galperin, Tatiana A. Gaidenko, Armen Y. Mulkidjanian, Michiko Nakano, Chester W. Price

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

MHYT, a new conserved protein domain with a likely signaling function, is described. This domain consists of six transmembrane segments, three of which contain conserved methionine, histidine, and tyrosine residues that are projected to lie near the outer face of the cytoplasmic membrane. In Synechocystis sp. PCC6803, this domain forms the N-terminus of the sensor histidine kinase Slr2098. In Pseudomonas aeruginosa and several other organisms, the MHYT domain forms the N-terminal part of a three-domain protein together with previously described GGDEF and EAL domains, both of which have been associated with signal transduction due to their presence in likely signaling proteins. In Bacillus subtilis YkoW protein, an additional PAS domain is found between the MHYT and GGDEF domains. A ykoW null mutant of B. subtilis did not exhibit any growth alterations, consistent with a non-essential, signaling role of this protein. A model of the membrane topology of the MHYT domain indicates that its conserved residues could coordinate one or two copper ions, suggesting a role in sensing oxygen, CO, or NO.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalFEMS Microbiology Letters
Volume205
Issue number1
DOIs
StatePublished - Nov 27 2001

Fingerprint

Bacillus subtilis
Membranes
Synechocystis
Proteins
Carbon Monoxide
Histidine
Methionine
Pseudomonas aeruginosa
Tyrosine
Copper
Signal Transduction
Cell Membrane
Ions
Oxygen
Growth
Protein Domains
Histidine Kinase

Keywords

  • Anaerobic growth
  • Genome analysis
  • Metal binding
  • Protein domain
  • Sequence conservation
  • Signal transduction

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Galperin, M. Y., Gaidenko, T. A., Mulkidjanian, A. Y., Nakano, M., & Price, C. W. (2001). MHYT, a new integral membrane sensor domain. FEMS Microbiology Letters, 205(1), 17-23. https://doi.org/10.1016/S0378-1097(01)00424-4

MHYT, a new integral membrane sensor domain. / Galperin, Michael Y.; Gaidenko, Tatiana A.; Mulkidjanian, Armen Y.; Nakano, Michiko; Price, Chester W.

In: FEMS Microbiology Letters, Vol. 205, No. 1, 27.11.2001, p. 17-23.

Research output: Contribution to journalArticle

Galperin, MY, Gaidenko, TA, Mulkidjanian, AY, Nakano, M & Price, CW 2001, 'MHYT, a new integral membrane sensor domain', FEMS Microbiology Letters, vol. 205, no. 1, pp. 17-23. https://doi.org/10.1016/S0378-1097(01)00424-4
Galperin MY, Gaidenko TA, Mulkidjanian AY, Nakano M, Price CW. MHYT, a new integral membrane sensor domain. FEMS Microbiology Letters. 2001 Nov 27;205(1):17-23. https://doi.org/10.1016/S0378-1097(01)00424-4
Galperin, Michael Y. ; Gaidenko, Tatiana A. ; Mulkidjanian, Armen Y. ; Nakano, Michiko ; Price, Chester W. / MHYT, a new integral membrane sensor domain. In: FEMS Microbiology Letters. 2001 ; Vol. 205, No. 1. pp. 17-23.
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