Mexneurin is a novel precursor of peptides in the central nervous system of rodents

Maura E. Matus-Ortega, Philippe Leff Gelman, Juan C. Calva-Nieves, Anabel Flores-Zamora, Alberto Salazar-Juárez, Carlos Alejandro Torner-Aguilar, Gerardo Gamba, Paola De Los Heros, Bonnie Peng, John E. Pintar, Heinrich S. Gompf, Charles Allen, Benito Antón-Palma

Research output: Contribution to journalLetter

3 Citations (Scopus)

Abstract

Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ-opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity-purified rabbit antisera raised against synthetic EMs in a whole-mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin-1 (an EM-like peptide), Mexneurin-2, and Mexneurin-3, a peptide which appears to be unrelated to EMs. RT-PCR analysis and in situ hybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin-1 and Mexneurin-3 peptides display biological activities in the mouse CNS.

Original languageEnglish (US)
Pages (from-to)1627-1636
Number of pages10
JournalFEBS Letters
Volume591
Issue number12
DOIs
StatePublished - Jun 1 2017

Fingerprint

Neurology
Rodentia
Central Nervous System
Peptides
Protein Precursors
Brain
DNA sequences
Opioid Receptors
Bioactivity
Gene Library
Names
In Situ Hybridization
Immune Sera
Assays
Rabbits
Ligands
Polymerase Chain Reaction
Messenger RNA

Keywords

  • endomorphin
  • opioid peptides
  • protein precursor

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Matus-Ortega, M. E., Leff Gelman, P., Calva-Nieves, J. C., Flores-Zamora, A., Salazar-Juárez, A., Torner-Aguilar, C. A., ... Antón-Palma, B. (2017). Mexneurin is a novel precursor of peptides in the central nervous system of rodents. FEBS Letters, 591(12), 1627-1636. https://doi.org/10.1002/1873-3468.12679

Mexneurin is a novel precursor of peptides in the central nervous system of rodents. / Matus-Ortega, Maura E.; Leff Gelman, Philippe; Calva-Nieves, Juan C.; Flores-Zamora, Anabel; Salazar-Juárez, Alberto; Torner-Aguilar, Carlos Alejandro; Gamba, Gerardo; De Los Heros, Paola; Peng, Bonnie; Pintar, John E.; Gompf, Heinrich S.; Allen, Charles; Antón-Palma, Benito.

In: FEBS Letters, Vol. 591, No. 12, 01.06.2017, p. 1627-1636.

Research output: Contribution to journalLetter

Matus-Ortega, ME, Leff Gelman, P, Calva-Nieves, JC, Flores-Zamora, A, Salazar-Juárez, A, Torner-Aguilar, CA, Gamba, G, De Los Heros, P, Peng, B, Pintar, JE, Gompf, HS, Allen, C & Antón-Palma, B 2017, 'Mexneurin is a novel precursor of peptides in the central nervous system of rodents', FEBS Letters, vol. 591, no. 12, pp. 1627-1636. https://doi.org/10.1002/1873-3468.12679
Matus-Ortega ME, Leff Gelman P, Calva-Nieves JC, Flores-Zamora A, Salazar-Juárez A, Torner-Aguilar CA et al. Mexneurin is a novel precursor of peptides in the central nervous system of rodents. FEBS Letters. 2017 Jun 1;591(12):1627-1636. https://doi.org/10.1002/1873-3468.12679
Matus-Ortega, Maura E. ; Leff Gelman, Philippe ; Calva-Nieves, Juan C. ; Flores-Zamora, Anabel ; Salazar-Juárez, Alberto ; Torner-Aguilar, Carlos Alejandro ; Gamba, Gerardo ; De Los Heros, Paola ; Peng, Bonnie ; Pintar, John E. ; Gompf, Heinrich S. ; Allen, Charles ; Antón-Palma, Benito. / Mexneurin is a novel precursor of peptides in the central nervous system of rodents. In: FEBS Letters. 2017 ; Vol. 591, No. 12. pp. 1627-1636.
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