TY - JOUR
T1 - Mexneurin is a novel precursor of peptides in the central nervous system of rodents
AU - Matus-Ortega, Maura E.
AU - Leff Gelman, Philippe
AU - Calva-Nieves, Juan C.
AU - Flores-Zamora, Anabel
AU - Salazar-Juárez, Alberto
AU - Torner-Aguilar, Carlos Alejandro
AU - Gamba, Gerardo
AU - De Los Heros, Paola
AU - Peng, Bonnie
AU - Pintar, John E.
AU - Gompf, Heinrich S.
AU - Allen, Charles N.
AU - Antón-Palma, Benito
N1 - Funding Information:
Maura Epifan?a Matus Ortega is a doctoral student from Programa de Doctorado en Ciencias Biom?dicas, Universidad Nacional Aut?noma de M?xico (UNAM) and received fellowship 197991 from CONACYT. This work was supported by the Fundaci?n Gonzalo R?o Arronte, IAP as well. The work was also supported by NIH grant NS036607 (CNA). We thank Dr. Jaime Garc?a-Mena for critically reading the manuscript and we thank the technical help of Norma Vazquez BsC.
Publisher Copyright:
© 2017 Federation of European Biochemical Societies
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ-opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity-purified rabbit antisera raised against synthetic EMs in a whole-mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin-1 (an EM-like peptide), Mexneurin-2, and Mexneurin-3, a peptide which appears to be unrelated to EMs. RT-PCR analysis and in situ hybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin-1 and Mexneurin-3 peptides display biological activities in the mouse CNS.
AB - Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ-opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity-purified rabbit antisera raised against synthetic EMs in a whole-mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin-1 (an EM-like peptide), Mexneurin-2, and Mexneurin-3, a peptide which appears to be unrelated to EMs. RT-PCR analysis and in situ hybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin-1 and Mexneurin-3 peptides display biological activities in the mouse CNS.
KW - endomorphin
KW - opioid peptides
KW - protein precursor
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U2 - 10.1002/1873-3468.12679
DO - 10.1002/1873-3468.12679
M3 - Article
C2 - 28504339
AN - SCOPUS:85028244415
SN - 0014-5793
VL - 591
SP - 1627
EP - 1636
JO - FEBS Letters
JF - FEBS Letters
IS - 12
ER -