TY - JOUR
T1 - Mexneurin is a novel precursor of peptides in the central nervous system of rodents
AU - Matus-Ortega, Maura E.
AU - Leff Gelman, Philippe
AU - Calva-Nieves, Juan C.
AU - Flores-Zamora, Anabel
AU - Salazar-Juárez, Alberto
AU - Torner-Aguilar, Carlos Alejandro
AU - Gamba, Gerardo
AU - De Los Heros, Paola
AU - Peng, Bonnie
AU - Pintar, John E.
AU - Gompf, Heinrich S.
AU - Allen, Charles N.
AU - Antón-Palma, Benito
N1 - Publisher Copyright:
© 2017 Federation of European Biochemical Societies
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ-opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity-purified rabbit antisera raised against synthetic EMs in a whole-mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin-1 (an EM-like peptide), Mexneurin-2, and Mexneurin-3, a peptide which appears to be unrelated to EMs. RT-PCR analysis and in situ hybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin-1 and Mexneurin-3 peptides display biological activities in the mouse CNS.
AB - Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ-opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity-purified rabbit antisera raised against synthetic EMs in a whole-mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin-1 (an EM-like peptide), Mexneurin-2, and Mexneurin-3, a peptide which appears to be unrelated to EMs. RT-PCR analysis and in situ hybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin-1 and Mexneurin-3 peptides display biological activities in the mouse CNS.
KW - endomorphin
KW - opioid peptides
KW - protein precursor
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U2 - 10.1002/1873-3468.12679
DO - 10.1002/1873-3468.12679
M3 - Article
C2 - 28504339
AN - SCOPUS:85028244415
SN - 0014-5793
VL - 591
SP - 1627
EP - 1636
JO - FEBS Letters
JF - FEBS Letters
IS - 12
ER -