Methodology for Detecting and Tracking Brain-Derived Neurotrophic Factor Complexes in Neurons Using Single Quantum Dots

Anke Vermehren-Schmaedick, Thomas Jacob, Tothu (Tania) Vu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We describe a methodological workflow for detecting and tracking single brain-derived neurotrophic factor (BDNF) receptor complexes in primary cultures of neurons. This methodology includes the preparation of BDNF-conjugated quantum dot fluorescent probes for sensitive detection of single BDNF complexes in fixed neurons as well as single-particle tracking (SPT) in live neurons. These methods are valuable for high-resolution localization and quantitation of low-abundance proteins in single neurons and for obtaining nanoscale-resolution, dynamic information of BDNF trafficking for long time durations (min) in live neurons.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages105-118
Number of pages14
DOIs
StatePublished - Jan 1 2019

Publication series

NameNeuromethods
Volume143
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • BDNF
  • Dynamics
  • Growth factor
  • Intracellular trafficking
  • Quantum dot
  • Single-particle tracking
  • TrkB

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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    Vermehren-Schmaedick, A., Jacob, T., & Vu, T. T. (2019). Methodology for Detecting and Tracking Brain-Derived Neurotrophic Factor Complexes in Neurons Using Single Quantum Dots. In Neuromethods (pp. 105-118). (Neuromethods; Vol. 143). Humana Press Inc.. https://doi.org/10.1007/7657_2018_11