@inbook{1ee44e3064f4421ebc45ea0de14ca625,
title = "Methodology for Detecting and Tracking Brain-Derived Neurotrophic Factor Complexes in Neurons Using Single Quantum Dots",
abstract = "We describe a methodological workflow for detecting and tracking single brain-derived neurotrophic factor (BDNF) receptor complexes in primary cultures of neurons. This methodology includes the preparation of BDNF-conjugated quantum dot fluorescent probes for sensitive detection of single BDNF complexes in fixed neurons as well as single-particle tracking (SPT) in live neurons. These methods are valuable for high-resolution localization and quantitation of low-abundance proteins in single neurons and for obtaining nanoscale-resolution, dynamic information of BDNF trafficking for long time durations (min) in live neurons.",
keywords = "BDNF, Dynamics, Growth factor, Intracellular trafficking, Quantum dot, Single-particle tracking, TrkB",
author = "Anke Vermehren-Schmaedick and Thomas Jacob and Vu, {Tania Q.}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media New York.",
year = "2019",
doi = "10.1007/7657_2018_11",
language = "English (US)",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "105--118",
booktitle = "Neuromethods",
}