Methamphetamine induces the release of endothelin

Jeong Woo Seo, Susan M. Jones, Trisha A. Hostetter, Jeffrey Iliff, G. Alexander West

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Methamphetamine is a potent psychostimulant drug of abuse that increases release and blocks reuptake of dopamine, producing intense euphoria, factors that may contribute to its widespread abuse. It also produces severe neurotoxicity resulting from oxidative stress, DNA damage, blood-brain barrier disruption, microgliosis, and mitochondrial dysfunction. Intracerebral hemorrhagic and ischemic stroke have been reported after intravenous and oral abuse of methamphetamine. Several studies have shown that methamphetamine causes vasoconstriction of vessels. This study investigates the effect of methamphetamine on endothelin-1 (ET-1) release in mouse brain endothelial cells by ELISA. ET-1 transcription as well as endothelial nitric oxide synthase (eNOS) activation and transcription were measured following methamphetamine treatment. We also examine the effect of methamphetamine on isolated cerebral arteriolar vessels from C57BL/6 mice. Penetrating middle cerebral arterioles were cannulated at both ends with a micropipette system. Methamphetamine was applied extraluminally, and the vascular response was investigated. Methamphetamine treatment of mouse brain endothelial cells resulted in ET-1 release and a transient increase in ET-1 message. The activity and transcription of eNOS were only slightly enhanced after 24hr of treatment with methamphetamine. In addition, methamphetamine caused significant vasoconstriction of isolated mouse intracerebral arterioles. The vasoconstrictive effect of methamphetamine was attenuated by coapplication of the endothelin receptor antagonist PD145065. These findings suggest that vasoconstriction induced by methamphetamine is mediated through the endothelin receptor and may involve an endothelin-dependent pathway.

Original languageEnglish (US)
Pages (from-to)170-178
Number of pages9
JournalJournal of Neuroscience Research
Volume94
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Methamphetamine
Endothelins
Endothelin-1
Vasoconstriction
Nitric Oxide Synthase Type III
Arterioles
Endothelial Cells
Endothelin Receptors
Brain
Street Drugs
Blood-Brain Barrier
Inbred C57BL Mouse
Transcriptional Activation
DNA Damage
Blood Vessels
Dopamine
Oxidative Stress
Therapeutics
Enzyme-Linked Immunosorbent Assay
Stroke

Keywords

  • Endothelial cells
  • Endothelin
  • Isolated intracerebral arterioles
  • Methamphetamine
  • Vasoconstriction

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Seo, J. W., Jones, S. M., Hostetter, T. A., Iliff, J., & West, G. A. (2016). Methamphetamine induces the release of endothelin. Journal of Neuroscience Research, 94(2), 170-178. https://doi.org/10.1002/jnr.23697

Methamphetamine induces the release of endothelin. / Seo, Jeong Woo; Jones, Susan M.; Hostetter, Trisha A.; Iliff, Jeffrey; West, G. Alexander.

In: Journal of Neuroscience Research, Vol. 94, No. 2, 01.02.2016, p. 170-178.

Research output: Contribution to journalArticle

Seo, JW, Jones, SM, Hostetter, TA, Iliff, J & West, GA 2016, 'Methamphetamine induces the release of endothelin', Journal of Neuroscience Research, vol. 94, no. 2, pp. 170-178. https://doi.org/10.1002/jnr.23697
Seo, Jeong Woo ; Jones, Susan M. ; Hostetter, Trisha A. ; Iliff, Jeffrey ; West, G. Alexander. / Methamphetamine induces the release of endothelin. In: Journal of Neuroscience Research. 2016 ; Vol. 94, No. 2. pp. 170-178.
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