Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

Le Ann Tiede, Peter S. Steyger, Michael G. Nichols, Richard Hallworth

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalBrain research
Volume1277
DOIs
StatePublished - Jun 24 2009

Keywords

  • Aminoglycoside
  • Gentamicin
  • Multi-photon confocal microscopy
  • NADH
  • Sensorineural hearing loss

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Metabolic imaging of the organ of corti - A window on cochlea bioenergetics'. Together they form a unique fingerprint.

  • Cite this