Metabolic heterogeneity among human monocytes and its modulation by PGE2

Louis Picker, H. V. Raff, M. E. Goldyne, J. D. Stobo

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Human peripheral blood monocytes (M∅) were fractionated on a discontinuous bovine serum albumin density gradient, and cells from each fraction was assayed for their specific activity of two enzymes, which represent correlates of M∅ activation or differentiation (5'-nucleotidase, acid phosphatase) and their synthesis of prostaglandin E2 (PGE2). On the basis of significant differences in these parameters, the monocytes could be divided into two broad populations. Low density cells demonstrated low specific activities of 5'-nucleotidase (5'N), high specific activities of acid phosphatase (APT'ase), and synthesized substantial amounts of PGE2. In contrast, high density cells demonstrated high specific activities of 5'-N, low specific activities of APT'ase, and synthesized meager amounts of PGE2. To determine if these biochemical differences served as stable markers of M∅ subpopulations, low and high density M∅ were individually cultured for 3 days, and changes in the metabolic parameters were assayed. Although 5'-N and APT'ase, increased among each population, the magnitude of this change could be modulated by PGE2. When each population was cultured in a concentration of PGE2 equivalent to that synthesized by the whole M∅ differences in 5'-N and APT'ase, remained as useful discriminators of the M∅ subpopulations. The pertinence of these findings to events involved in M∅ activation and differentiation as well as a possible role for PGE2 in modulating these events is discussed.

Original languageEnglish (US)
Pages (from-to)2557-2562
Number of pages6
JournalJournal of Immunology
Volume124
Issue number6
StatePublished - 1980
Externally publishedYes

Fingerprint

Dinoprostone
Acid Phosphatase
Monocytes
5'-Nucleotidase
Cell Count
Population
Bovine Serum Albumin
Enzymes

ASJC Scopus subject areas

  • Immunology

Cite this

Picker, L., Raff, H. V., Goldyne, M. E., & Stobo, J. D. (1980). Metabolic heterogeneity among human monocytes and its modulation by PGE2 . Journal of Immunology, 124(6), 2557-2562.

Metabolic heterogeneity among human monocytes and its modulation by PGE2 . / Picker, Louis; Raff, H. V.; Goldyne, M. E.; Stobo, J. D.

In: Journal of Immunology, Vol. 124, No. 6, 1980, p. 2557-2562.

Research output: Contribution to journalArticle

Picker, L, Raff, HV, Goldyne, ME & Stobo, JD 1980, 'Metabolic heterogeneity among human monocytes and its modulation by PGE2 ', Journal of Immunology, vol. 124, no. 6, pp. 2557-2562.
Picker, Louis ; Raff, H. V. ; Goldyne, M. E. ; Stobo, J. D. / Metabolic heterogeneity among human monocytes and its modulation by PGE2 . In: Journal of Immunology. 1980 ; Vol. 124, No. 6. pp. 2557-2562.
@article{8fdc1c3ffdae497a9e927f15844978a8,
title = "Metabolic heterogeneity among human monocytes and its modulation by PGE2",
abstract = "Human peripheral blood monocytes (M∅) were fractionated on a discontinuous bovine serum albumin density gradient, and cells from each fraction was assayed for their specific activity of two enzymes, which represent correlates of M∅ activation or differentiation (5'-nucleotidase, acid phosphatase) and their synthesis of prostaglandin E2 (PGE2). On the basis of significant differences in these parameters, the monocytes could be divided into two broad populations. Low density cells demonstrated low specific activities of 5'-nucleotidase (5'N), high specific activities of acid phosphatase (APT'ase), and synthesized substantial amounts of PGE2. In contrast, high density cells demonstrated high specific activities of 5'-N, low specific activities of APT'ase, and synthesized meager amounts of PGE2. To determine if these biochemical differences served as stable markers of M∅ subpopulations, low and high density M∅ were individually cultured for 3 days, and changes in the metabolic parameters were assayed. Although 5'-N and APT'ase, increased among each population, the magnitude of this change could be modulated by PGE2. When each population was cultured in a concentration of PGE2 equivalent to that synthesized by the whole M∅ differences in 5'-N and APT'ase, remained as useful discriminators of the M∅ subpopulations. The pertinence of these findings to events involved in M∅ activation and differentiation as well as a possible role for PGE2 in modulating these events is discussed.",
author = "Louis Picker and Raff, {H. V.} and Goldyne, {M. E.} and Stobo, {J. D.}",
year = "1980",
language = "English (US)",
volume = "124",
pages = "2557--2562",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "6",

}

TY - JOUR

T1 - Metabolic heterogeneity among human monocytes and its modulation by PGE2

AU - Picker, Louis

AU - Raff, H. V.

AU - Goldyne, M. E.

AU - Stobo, J. D.

PY - 1980

Y1 - 1980

N2 - Human peripheral blood monocytes (M∅) were fractionated on a discontinuous bovine serum albumin density gradient, and cells from each fraction was assayed for their specific activity of two enzymes, which represent correlates of M∅ activation or differentiation (5'-nucleotidase, acid phosphatase) and their synthesis of prostaglandin E2 (PGE2). On the basis of significant differences in these parameters, the monocytes could be divided into two broad populations. Low density cells demonstrated low specific activities of 5'-nucleotidase (5'N), high specific activities of acid phosphatase (APT'ase), and synthesized substantial amounts of PGE2. In contrast, high density cells demonstrated high specific activities of 5'-N, low specific activities of APT'ase, and synthesized meager amounts of PGE2. To determine if these biochemical differences served as stable markers of M∅ subpopulations, low and high density M∅ were individually cultured for 3 days, and changes in the metabolic parameters were assayed. Although 5'-N and APT'ase, increased among each population, the magnitude of this change could be modulated by PGE2. When each population was cultured in a concentration of PGE2 equivalent to that synthesized by the whole M∅ differences in 5'-N and APT'ase, remained as useful discriminators of the M∅ subpopulations. The pertinence of these findings to events involved in M∅ activation and differentiation as well as a possible role for PGE2 in modulating these events is discussed.

AB - Human peripheral blood monocytes (M∅) were fractionated on a discontinuous bovine serum albumin density gradient, and cells from each fraction was assayed for their specific activity of two enzymes, which represent correlates of M∅ activation or differentiation (5'-nucleotidase, acid phosphatase) and their synthesis of prostaglandin E2 (PGE2). On the basis of significant differences in these parameters, the monocytes could be divided into two broad populations. Low density cells demonstrated low specific activities of 5'-nucleotidase (5'N), high specific activities of acid phosphatase (APT'ase), and synthesized substantial amounts of PGE2. In contrast, high density cells demonstrated high specific activities of 5'-N, low specific activities of APT'ase, and synthesized meager amounts of PGE2. To determine if these biochemical differences served as stable markers of M∅ subpopulations, low and high density M∅ were individually cultured for 3 days, and changes in the metabolic parameters were assayed. Although 5'-N and APT'ase, increased among each population, the magnitude of this change could be modulated by PGE2. When each population was cultured in a concentration of PGE2 equivalent to that synthesized by the whole M∅ differences in 5'-N and APT'ase, remained as useful discriminators of the M∅ subpopulations. The pertinence of these findings to events involved in M∅ activation and differentiation as well as a possible role for PGE2 in modulating these events is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0018823623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018823623&partnerID=8YFLogxK

M3 - Article

VL - 124

SP - 2557

EP - 2562

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 6

ER -