Metabolic changes in glucose transporter-deficient Leishmania mexicana and parasite virulence

Dayana Rodríguez-Contreras, Scott Landfear

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Leishmania mexicana are parasitic protozoa that express a variety of glycoconjugates that play important roles in their biology as well as the storage carbohydrate β-mannan, which is an essential virulence factor for survival of intracellular amastigote forms in the mammalian host. Glucose transporter null mutants, which are viable as insect form promastigotes but not as amastigotes, do not take up glucose and other hexoses but are still able to synthesize these glycoconjugates and β-mannan, although at reduced levels. Synthesis of these carbohydrate-containing macromolecules could be accounted for by incorporation of non-carbohydrate precursors into carbohydrates by gluconeogenesis. However, the significantly reduced level of the virulence factor β-mannan in the glucose transporter null mutants compared with wild-type parasites may contribute to the non-viability of these null mutants in the disease-causing amastigote stage of the life cycle.

Original languageEnglish (US)
Pages (from-to)20068-20076
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number29
DOIs
StatePublished - Jul 21 2006

Fingerprint

Mannans
Facilitative Glucose Transport Proteins
Virulence
Parasites
Glycoconjugates
Carbohydrates
Virulence Factors
Leishmania mexicana
Protozoa
Hexoses
Gluconeogenesis
Life Cycle Stages
Macromolecules
Insects
Life cycle
Glucose
Leishmania glucose transporter

ASJC Scopus subject areas

  • Biochemistry

Cite this

Metabolic changes in glucose transporter-deficient Leishmania mexicana and parasite virulence. / Rodríguez-Contreras, Dayana; Landfear, Scott.

In: Journal of Biological Chemistry, Vol. 281, No. 29, 21.07.2006, p. 20068-20076.

Research output: Contribution to journalArticle

@article{a4aa3dabbf36455ebfc1b7668a3a34fa,
title = "Metabolic changes in glucose transporter-deficient Leishmania mexicana and parasite virulence",
abstract = "Leishmania mexicana are parasitic protozoa that express a variety of glycoconjugates that play important roles in their biology as well as the storage carbohydrate β-mannan, which is an essential virulence factor for survival of intracellular amastigote forms in the mammalian host. Glucose transporter null mutants, which are viable as insect form promastigotes but not as amastigotes, do not take up glucose and other hexoses but are still able to synthesize these glycoconjugates and β-mannan, although at reduced levels. Synthesis of these carbohydrate-containing macromolecules could be accounted for by incorporation of non-carbohydrate precursors into carbohydrates by gluconeogenesis. However, the significantly reduced level of the virulence factor β-mannan in the glucose transporter null mutants compared with wild-type parasites may contribute to the non-viability of these null mutants in the disease-causing amastigote stage of the life cycle.",
author = "Dayana Rodr{\'i}guez-Contreras and Scott Landfear",
year = "2006",
month = "7",
day = "21",
doi = "10.1074/jbc.M603265200",
language = "English (US)",
volume = "281",
pages = "20068--20076",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "29",

}

TY - JOUR

T1 - Metabolic changes in glucose transporter-deficient Leishmania mexicana and parasite virulence

AU - Rodríguez-Contreras, Dayana

AU - Landfear, Scott

PY - 2006/7/21

Y1 - 2006/7/21

N2 - Leishmania mexicana are parasitic protozoa that express a variety of glycoconjugates that play important roles in their biology as well as the storage carbohydrate β-mannan, which is an essential virulence factor for survival of intracellular amastigote forms in the mammalian host. Glucose transporter null mutants, which are viable as insect form promastigotes but not as amastigotes, do not take up glucose and other hexoses but are still able to synthesize these glycoconjugates and β-mannan, although at reduced levels. Synthesis of these carbohydrate-containing macromolecules could be accounted for by incorporation of non-carbohydrate precursors into carbohydrates by gluconeogenesis. However, the significantly reduced level of the virulence factor β-mannan in the glucose transporter null mutants compared with wild-type parasites may contribute to the non-viability of these null mutants in the disease-causing amastigote stage of the life cycle.

AB - Leishmania mexicana are parasitic protozoa that express a variety of glycoconjugates that play important roles in their biology as well as the storage carbohydrate β-mannan, which is an essential virulence factor for survival of intracellular amastigote forms in the mammalian host. Glucose transporter null mutants, which are viable as insect form promastigotes but not as amastigotes, do not take up glucose and other hexoses but are still able to synthesize these glycoconjugates and β-mannan, although at reduced levels. Synthesis of these carbohydrate-containing macromolecules could be accounted for by incorporation of non-carbohydrate precursors into carbohydrates by gluconeogenesis. However, the significantly reduced level of the virulence factor β-mannan in the glucose transporter null mutants compared with wild-type parasites may contribute to the non-viability of these null mutants in the disease-causing amastigote stage of the life cycle.

UR - http://www.scopus.com/inward/record.url?scp=33746000107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746000107&partnerID=8YFLogxK

U2 - 10.1074/jbc.M603265200

DO - 10.1074/jbc.M603265200

M3 - Article

C2 - 16707495

AN - SCOPUS:33746000107

VL - 281

SP - 20068

EP - 20076

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 29

ER -