Metabolic Activation of the HOG MAP Kinase Pathway by Snf1/AMPK Regulates Lipid Signaling at the Golgi

Hailan Piao, John Maclean Freed, Peter Mayinger

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Phosphatidylinositol-4-phosphate (PI(4)P) is an important regulator of Golgi function. Metabolic regulation of Golgi PI(4)P requires the lipid phosphatase Sac1 that translocates between endoplasmic reticulum (ER) and Golgi membranes. Localization of Sac1 responds to changes in glucose levels, yet the upstream signaling pathways that regulate Sac1 traffic are unknown. Here, we report that mitogen-activated protein kinase (MAPK) Hog1 transmits glucose signals to the Golgi and regulates localization of Sac1. We find that Hog1 is rapidly activated by both glucose starvation and glucose stimulation, which is independent of the well-characterized response to osmotic stress but requires the upstream element Ssk1 and is controlled by Snf1, the yeast homolog of AMP-activated kinase (AMPK). Elimination of either Hog1 or Snf1 slows glucose-induced translocation of Sac1 lipid phosphatase from the Golgi to the ER and thus delays PI(4)P accumulation at the Golgi. We conclude that a novel cross-talk between the HOG pathway and Snf1/AMPK is required for the metabolic control of lipid signaling at the Golgi.

Original languageEnglish (US)
Pages (from-to)1522-1531
Number of pages10
JournalTraffic
Volume13
Issue number11
DOIs
StatePublished - Nov 2012

Fingerprint

AMP-Activated Protein Kinases
Phosphotransferases
Chemical activation
Lipids
Glucose
Phosphoric Monoester Hydrolases
Endoplasmic Reticulum
Osmoregulation
Starvation
Mitogen-Activated Protein Kinases
Yeast
Yeasts
Metabolic Activation
SNF1-related protein kinases
Membranes
phosphatidylinositol 4-phosphate

Keywords

  • Glucose signaling
  • Golgi
  • Hog1 MAP kinase
  • Phosphoinositides
  • Sac1
  • Snf1/AMPK

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Metabolic Activation of the HOG MAP Kinase Pathway by Snf1/AMPK Regulates Lipid Signaling at the Golgi. / Piao, Hailan; Maclean Freed, John; Mayinger, Peter.

In: Traffic, Vol. 13, No. 11, 11.2012, p. 1522-1531.

Research output: Contribution to journalArticle

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