Membrane type-1 matrix metalloproteinase confers aneuploidy and tumorigenicity on mammary epithelial cells

Vladislav S. Golubkov, Alexey V. Chekanov, Alexey Y. Savinov, Dmitri Rozanov, Natalya V. Golubkova, Alex Y. Strongin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

An elevated expression of membrane type-1 matrix metalloproteinase (MT1-MMP) is closely associated with multiple malignancies. Recently, we discovered that recycled MT1-MMP was trafficked along the tubulin cytoskeleton into the centrosomal compartment and cleaved the integral centrosomal protein pericentrin-2. These events correlated with the induction of chromosome instability and aneuploidy in nonmalignant Madine-Darby canine kidney cells. Accordingly, we hypothesized that MT1-MMP is an oncogene that promotes malignant transformation of normal cells rather than just an enzyme that supports growth of preexisting tumors. To prove our hypothesis, we transfected normal 184B5 human mammary epithelial cells with MT1-MMP (184B5-MT1 cells). MT1-MMP was colocalized with pericentrin in the centrosomal compartment and especially in the midbody of dividing cells. 184B5-MT1 cells acquired the ability to activate MMP-2, to cleave pericentrin, and to invade the Matrigel matrix. 184B5-MT1 cells exhibited aneuploidy, and they were efficient in generating tumors in the orthotopic xenograft model in immunodeficient mice. Because of the absence of tumor angiogenesis and the resulting insufficient blood supply, the tumors then regressed with significant accompanying necrosis. Gene array studies confirmed a significant upregulation of oncogenes and tumorigenic genes but not the angiogenesis-promoting genes in 184B5-MT1 cells. We believe that our data point to a novel function of MT1-MMP in the initial stages of malignant transformation and to new and hitherto unknown transition mechanism from normalcy to malignancy.

Original languageEnglish (US)
Pages (from-to)10460-10465
Number of pages6
JournalCancer Research
Volume66
Issue number21
DOIs
StatePublished - Nov 1 2006
Externally publishedYes

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Matrix Metalloproteinase 14
Aneuploidy
Breast
Epithelial Cells
Neoplasms
Oncogenes
Genes
Chromosomal Instability
Tubulin
Cytoskeleton
Matrix Metalloproteinases
Heterografts
Canidae
Necrosis
Up-Regulation
Kidney
Enzymes
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Membrane type-1 matrix metalloproteinase confers aneuploidy and tumorigenicity on mammary epithelial cells. / Golubkov, Vladislav S.; Chekanov, Alexey V.; Savinov, Alexey Y.; Rozanov, Dmitri; Golubkova, Natalya V.; Strongin, Alex Y.

In: Cancer Research, Vol. 66, No. 21, 01.11.2006, p. 10460-10465.

Research output: Contribution to journalArticle

Golubkov, Vladislav S. ; Chekanov, Alexey V. ; Savinov, Alexey Y. ; Rozanov, Dmitri ; Golubkova, Natalya V. ; Strongin, Alex Y. / Membrane type-1 matrix metalloproteinase confers aneuploidy and tumorigenicity on mammary epithelial cells. In: Cancer Research. 2006 ; Vol. 66, No. 21. pp. 10460-10465.
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