TY - JOUR
T1 - Membrane lipids tune synaptic transmission by direct modulation of presynaptic potassium channels
AU - Carta, Mario
AU - Lanore, Frederic
AU - Rebola, Nelson
AU - Szabo, Zsolt
AU - Da Silva, Silvia Viana
AU - Lourenço, Joana
AU - Verraes, Agathe
AU - Nadler, André
AU - Schultz, Carsten
AU - Blanchet, Christophe
AU - Mulle, Christophe
N1 - Funding Information:
M.C. was supported by an EIF Fellowship (project name KARTRAF), and F.L. was supported by SYNSCAFF, the Conseil Regional of Aquitaine, the Fondation pour le Recherche Medicale, the ANR ASD/LD. Z.S. was supported by a stipend from Dr. Alan Fine (Dalhousie University). C.S. and A.N. acknowledge funding of the ESF EuroMembrane program (TraPPs). We thank H. Alle for comments on the manuscript and for valuable suggestions on the MfB recording, J. Mellor for sharing with us the natural sequence of spiking activity, A. Frick for suggestions on Kv currents recordings, and R.A. Silver, G. Marsicano, and J. Barhanin for comments on the manuscript.
PY - 2014/2/19
Y1 - 2014/2/19
N2 - Voltage-gated potassium (Kv) channels are involved in action potential (AP) repolarization in excitable cells. Exogenous application of membrane-derived lipids, such as arachidonic acid (AA), regulates the gating of Kv channels. Whether membrane-derived lipids released under physiological conditions have an impact on neuronal coding through this mechanism is unknown. We show that AA released in anactivity-dependent manner from postsynaptic hippocampal CA3 pyramidal cells acts as retrograde messenger, inducing a robust facilitation of mossy fiber (Mf) synaptic transmission over several minutes. AA acts by broadening presynaptic APs through thedirect modulation of Kv channels. This form of short-term plasticity can be triggered when postsynaptic cell fires with physiologically relevant patterns and sets the threshold for the induction of the presynaptic form of long-term potentiation (LTP) at hippocampal Mf synapses. Hence, direct modulation ofpresynaptic Kv channels by activity-dependent release of lipids serves as a physiological mechanism for tuning synaptic transmission.
AB - Voltage-gated potassium (Kv) channels are involved in action potential (AP) repolarization in excitable cells. Exogenous application of membrane-derived lipids, such as arachidonic acid (AA), regulates the gating of Kv channels. Whether membrane-derived lipids released under physiological conditions have an impact on neuronal coding through this mechanism is unknown. We show that AA released in anactivity-dependent manner from postsynaptic hippocampal CA3 pyramidal cells acts as retrograde messenger, inducing a robust facilitation of mossy fiber (Mf) synaptic transmission over several minutes. AA acts by broadening presynaptic APs through thedirect modulation of Kv channels. This form of short-term plasticity can be triggered when postsynaptic cell fires with physiologically relevant patterns and sets the threshold for the induction of the presynaptic form of long-term potentiation (LTP) at hippocampal Mf synapses. Hence, direct modulation ofpresynaptic Kv channels by activity-dependent release of lipids serves as a physiological mechanism for tuning synaptic transmission.
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U2 - 10.1016/j.neuron.2013.12.028
DO - 10.1016/j.neuron.2013.12.028
M3 - Article
C2 - 24486086
AN - SCOPUS:84896689929
SN - 0896-6273
VL - 81
SP - 787
EP - 799
JO - Neuron
JF - Neuron
IS - 4
ER -