Membrane dynamics of cleavage furrow closure in Xenopus laevis

Michael Danilchik, Elizabeth E. Brown

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Epithelial membrane polarity develops early in Xenopus development, with membrane inserted along the earliest cleavage furrows by means of localized exocytosis. The added surface constitutes a new basolateral domain important for early morphogenesis. This basolateral surface becomes isolated from the outside by furrow closure, a zippering of adjacent apical-basolateral margins. Time-lapse microscopy of membrane-labeled embryos revealed two distinct kinds of protrusive activity in furrow closure. Early in furrowing, protrusive activity was associated with purse-string contractility along the apical-basolateral margins. Later in furrow progression, a basolateral protrusive zone developed entirely within the new membrane domain, with long motile filopodia extending in contractile bands from the exposed surfaces. Filopodia interacting with opposing cell surfaces across the cleavage furrow appeared to mediate blastomere-blastomere adhesion, contact spreading and lamellipodial protrusion. Interference with these dynamic activities prevented furrow closure, indicating a basic role for both marginal and basolateral protrusive activities in early embryogenesis.

Original languageEnglish (US)
Pages (from-to)565-579
Number of pages15
JournalDevelopmental Dynamics
Volume237
Issue number3
DOIs
StatePublished - Mar 2008

Fingerprint

Xenopus laevis
Blastomeres
Pseudopodia
Membranes
Exocytosis
Xenopus
Morphogenesis
Embryonic Development
Microscopy
Embryonic Structures

Keywords

  • Cleavage furrow
  • Contractile ring
  • Embryo
  • Filopodia
  • Furrow closure
  • Lamellipodia
  • Xenopus
  • Zippering

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Membrane dynamics of cleavage furrow closure in Xenopus laevis. / Danilchik, Michael; Brown, Elizabeth E.

In: Developmental Dynamics, Vol. 237, No. 3, 03.2008, p. 565-579.

Research output: Contribution to journalArticle

Danilchik, Michael ; Brown, Elizabeth E. / Membrane dynamics of cleavage furrow closure in Xenopus laevis. In: Developmental Dynamics. 2008 ; Vol. 237, No. 3. pp. 565-579.
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