Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation

Amanda Cobos-Correa, Johanna B. Trojanek, Stefanie Diemer, Marcus A. Mall, Carsten Schultz

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.

Original languageEnglish (US)
Pages (from-to)628-630
Number of pages3
JournalNature Chemical Biology
Volume5
Issue number9
DOIs
StatePublished - Sep 2009

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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