Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields

J. M. Lee, F. Stormshak, J. M. Thompson, P. Thinesen, L. J. Painter, E. G. Olenchek, David Hess, R. Forbes, D. L. Foster

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

This study determined whether chronic exposure of female lambs to the electric and magnetic fields (EMF) of a high voltage transmission line can alter pineal secretion of melatonin and the normal occurrence of puberty. Twenty female Suffolk lambs were assigned randomly in equal numbers to a control and a treatment group. Treatment from 2 to 10 mo of age consisted of continuous exposure within the electrical environment of a 500-kV transmission line (mean electric field 6 kV/m, mean magnetic field 40 mG). Treated lambs were penned directly beneath the transmission line; control lambs were maintained in a pen of similar construction 229 m from the line where EMF were at ambient levels (mean electric field <10 V/m, mean magnetic field <0.3 mG). Melatonin was analyzed by RIA in serum of blood samples collected at 0.5-3-h intervals over eight 48-h periods. To assess attainment of puberty, serum concentrations of progesterone were determined by RIA from blood samples collected twice weekly beginning at 19 wk of age. Concentrations of circulating melatonin in control and treated lambs were low during daylight hours and increased during nighttime hours. The characteristic pattern of melatonin secretion during nighttime (amplitude, phase, and duration) did not differ between control and treatment groups. Age at puberty and number of subsequent estrous cycles also did not differ between groups. These data suggest that chronic exposure of developing female sheep to 60-Hz environmental EMF does not affect the mechanisms underlying the generation of the circadian pattern of melatonin secretion or the mechanisms involved in the onset of reproductive activity.

Original languageEnglish (US)
Pages (from-to)857-864
Number of pages8
JournalBiology of Reproduction
Volume49
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Melatonin
Magnetic Fields
Puberty
Estrous Cycle
Serum
Progesterone
Sheep
Control Groups

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Lee, J. M., Stormshak, F., Thompson, J. M., Thinesen, P., Painter, L. J., Olenchek, E. G., ... Foster, D. L. (1993). Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields. Biology of Reproduction, 49(4), 857-864.

Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields. / Lee, J. M.; Stormshak, F.; Thompson, J. M.; Thinesen, P.; Painter, L. J.; Olenchek, E. G.; Hess, David; Forbes, R.; Foster, D. L.

In: Biology of Reproduction, Vol. 49, No. 4, 1993, p. 857-864.

Research output: Contribution to journalArticle

Lee, JM, Stormshak, F, Thompson, JM, Thinesen, P, Painter, LJ, Olenchek, EG, Hess, D, Forbes, R & Foster, DL 1993, 'Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields', Biology of Reproduction, vol. 49, no. 4, pp. 857-864.
Lee JM, Stormshak F, Thompson JM, Thinesen P, Painter LJ, Olenchek EG et al. Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields. Biology of Reproduction. 1993;49(4):857-864.
Lee, J. M. ; Stormshak, F. ; Thompson, J. M. ; Thinesen, P. ; Painter, L. J. ; Olenchek, E. G. ; Hess, David ; Forbes, R. ; Foster, D. L. / Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields. In: Biology of Reproduction. 1993 ; Vol. 49, No. 4. pp. 857-864.
@article{34323c722af14dcebff09225180e7cfd,
title = "Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields",
abstract = "This study determined whether chronic exposure of female lambs to the electric and magnetic fields (EMF) of a high voltage transmission line can alter pineal secretion of melatonin and the normal occurrence of puberty. Twenty female Suffolk lambs were assigned randomly in equal numbers to a control and a treatment group. Treatment from 2 to 10 mo of age consisted of continuous exposure within the electrical environment of a 500-kV transmission line (mean electric field 6 kV/m, mean magnetic field 40 mG). Treated lambs were penned directly beneath the transmission line; control lambs were maintained in a pen of similar construction 229 m from the line where EMF were at ambient levels (mean electric field <10 V/m, mean magnetic field <0.3 mG). Melatonin was analyzed by RIA in serum of blood samples collected at 0.5-3-h intervals over eight 48-h periods. To assess attainment of puberty, serum concentrations of progesterone were determined by RIA from blood samples collected twice weekly beginning at 19 wk of age. Concentrations of circulating melatonin in control and treated lambs were low during daylight hours and increased during nighttime hours. The characteristic pattern of melatonin secretion during nighttime (amplitude, phase, and duration) did not differ between control and treatment groups. Age at puberty and number of subsequent estrous cycles also did not differ between groups. These data suggest that chronic exposure of developing female sheep to 60-Hz environmental EMF does not affect the mechanisms underlying the generation of the circadian pattern of melatonin secretion or the mechanisms involved in the onset of reproductive activity.",
author = "Lee, {J. M.} and F. Stormshak and Thompson, {J. M.} and P. Thinesen and Painter, {L. J.} and Olenchek, {E. G.} and David Hess and R. Forbes and Foster, {D. L.}",
year = "1993",
language = "English (US)",
volume = "49",
pages = "857--864",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Melatonin secretion and puberty in female lambs exposed to environmental electric and magnetic fields

AU - Lee, J. M.

AU - Stormshak, F.

AU - Thompson, J. M.

AU - Thinesen, P.

AU - Painter, L. J.

AU - Olenchek, E. G.

AU - Hess, David

AU - Forbes, R.

AU - Foster, D. L.

PY - 1993

Y1 - 1993

N2 - This study determined whether chronic exposure of female lambs to the electric and magnetic fields (EMF) of a high voltage transmission line can alter pineal secretion of melatonin and the normal occurrence of puberty. Twenty female Suffolk lambs were assigned randomly in equal numbers to a control and a treatment group. Treatment from 2 to 10 mo of age consisted of continuous exposure within the electrical environment of a 500-kV transmission line (mean electric field 6 kV/m, mean magnetic field 40 mG). Treated lambs were penned directly beneath the transmission line; control lambs were maintained in a pen of similar construction 229 m from the line where EMF were at ambient levels (mean electric field <10 V/m, mean magnetic field <0.3 mG). Melatonin was analyzed by RIA in serum of blood samples collected at 0.5-3-h intervals over eight 48-h periods. To assess attainment of puberty, serum concentrations of progesterone were determined by RIA from blood samples collected twice weekly beginning at 19 wk of age. Concentrations of circulating melatonin in control and treated lambs were low during daylight hours and increased during nighttime hours. The characteristic pattern of melatonin secretion during nighttime (amplitude, phase, and duration) did not differ between control and treatment groups. Age at puberty and number of subsequent estrous cycles also did not differ between groups. These data suggest that chronic exposure of developing female sheep to 60-Hz environmental EMF does not affect the mechanisms underlying the generation of the circadian pattern of melatonin secretion or the mechanisms involved in the onset of reproductive activity.

AB - This study determined whether chronic exposure of female lambs to the electric and magnetic fields (EMF) of a high voltage transmission line can alter pineal secretion of melatonin and the normal occurrence of puberty. Twenty female Suffolk lambs were assigned randomly in equal numbers to a control and a treatment group. Treatment from 2 to 10 mo of age consisted of continuous exposure within the electrical environment of a 500-kV transmission line (mean electric field 6 kV/m, mean magnetic field 40 mG). Treated lambs were penned directly beneath the transmission line; control lambs were maintained in a pen of similar construction 229 m from the line where EMF were at ambient levels (mean electric field <10 V/m, mean magnetic field <0.3 mG). Melatonin was analyzed by RIA in serum of blood samples collected at 0.5-3-h intervals over eight 48-h periods. To assess attainment of puberty, serum concentrations of progesterone were determined by RIA from blood samples collected twice weekly beginning at 19 wk of age. Concentrations of circulating melatonin in control and treated lambs were low during daylight hours and increased during nighttime hours. The characteristic pattern of melatonin secretion during nighttime (amplitude, phase, and duration) did not differ between control and treatment groups. Age at puberty and number of subsequent estrous cycles also did not differ between groups. These data suggest that chronic exposure of developing female sheep to 60-Hz environmental EMF does not affect the mechanisms underlying the generation of the circadian pattern of melatonin secretion or the mechanisms involved in the onset of reproductive activity.

UR - http://www.scopus.com/inward/record.url?scp=0027197512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027197512&partnerID=8YFLogxK

M3 - Article

C2 - 8218652

AN - SCOPUS:0027197512

VL - 49

SP - 857

EP - 864

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -